利用组织芯片高通量筛选脑膜瘤生物标志物。

PubMed ID
发表日期 2005年Jul月

原始出处 神经肿瘤学杂志
Journal of neuro-oncology
作者 Lusis  Eriks A  Chicoine  Michael R  Perry  Arie 

文献标题 利用组织芯片高通量筛选脑膜瘤生物标志物。
High throughput screening of meningioma biomarkers using a tissue microarray.

文献摘要

脑膜瘤是一种组织学和临床上具有多样性的中枢神经系统肿瘤,其分化和进展的免疫组化标记物很少。因此,我们使用高通量组织微阵列免疫组织化学(TMA-IHC)研究了一组潜在有用的脑膜瘤相关生物标记物,其中TMA包括9个血管外皮细胞瘤(HPC)和41个脑膜瘤,跨越所有等级,以及两个非典型脑膜瘤亚群,根据临床行为分层。所用抗体为孕酮受体(PR)、上皮膜抗原(EMA)、组织蛋白酶D、E-钙粘蛋白、血小板衍生生长因子(PDGF)受体β、PDGF-BB配体、生存素、上皮生长因子受体(EGFR)和血管内皮生长因子(VEGF)。在大多数情况下,肿瘤阳性率与以前报道的使用全断面IHC的频率相似。EMA、E-cadherin和PDGFRβ染色模式将间变性脑膜瘤与HPCs区分开来(分别为P<0.001、P=0.02和P=0.015)。在先前的研究中,PR和组织蛋白酶D的表达与肿瘤分级成反比。然而,PR和EGFR在有症状、手术切除的良性脑膜瘤和尸检发现的偶发性脑膜瘤中也有不同的表达。我们的结论是:(1)TMA-IHC是快速评估脑膜瘤生物标志物的一种准确有效的方法;(2)EMA、E-cadherin和PDGFR-beta有助于区分间变性脑膜瘤和HPC;(3)偶发性脑膜瘤的表达模式与手术切除的症状性脑膜瘤略有不同。


Meningiomas are histologically and clinically diverse CNS neoplasms with few available immunohistochemical markers of differentiation and progression. Therefore, we investigated a panel of potentially useful meningioma-associated biomarkers using high throughput tissue microarray immunohistochemistry (TMA-IHC) with a TMA that includes 9 hemangiopericytomas (HPCs) and 41 meningiomas spanning all grades, as well as two subsets of atypical meningiomas, stratified according to clinical behavior. Antibodies utilized were progesterone receptor (PR), epithelial membrane antigen (EMA), cathepsin D, E-cadherin, platelet derived growth factor (PDGF) receptor beta, PDGF BB ligand, survivin, epithelial growth factor receptor (EGFR), and vascular endothelial growth factor (VEGF). In most cases, frequencies of tumor positivity were similar to those previously reported using whole section IHC. EMA, E-cadherin, and PDGFR-beta staining patterns distinguished the anaplastic meningiomas from the HPCs (P < 0.001, P = 0.02, P = 0.015, respectively). As in prior studies, PR and cathepsin D expression were inversely proportional to tumor grade. However, PR and EGFR were also differentially expressed between symptomatic, surgically resected benign meningiomas and incidental meningiomas found at autopsy. We conclude that (1) TMA-IHC is an accurate and efficient way to rapidly assess biomarkers in meningeal tumors, (2) EMA, E-cadherin, and PDGFR-beta are useful in distinguishing anaplastic meningiomas from HPCs, and (3) the expression patterns for incidental meningiomas differ slightly from their surgically resected symptomatic counterparts.


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