脑膜瘤中β-catenin/E-cadherin表达的改变。

PubMed ID
发表日期 2006年Aug月

原始出处 组织病理学
Histopathology
作者 Brunner  E C  Romeike  B F M  Jung  M  Comtesse  N  Meese  E 

文献标题 脑膜瘤中β-catenin/E-cadherin表达的改变。
Altered expression of beta-catenin/E-cadherin in meningiomas.

文献摘要 AIMS

脑膜瘤通常是生长缓慢的良性肿瘤,约占原发性颅内肿瘤的20%。脑膜瘤发生的标志是22号染色体的丢失,包括2型神经纤维瘤病(NF2)基因杂合性的丢失。NF2编码的蛋白merlin似乎通过控制钙粘蛋白介导的细胞-细胞粘附发挥肿瘤抑制基因的作用。E-钙粘蛋白细胞粘附系统包括间接连接钙粘蛋白和肌动蛋白丝的β-连环蛋白。本研究旨在分析E-cadherin和β-catenin在脑膜瘤中的表达及亚细胞定位,包括不同组织形态亚型和不同世界卫生组织(WHO)分级的脑膜瘤。

METHODS AND RESULTS

免疫组化分析显示34%的脑膜瘤细胞膜缺乏E-cadherin表达,与WHO分级无关。79%的脑膜瘤发生膜β-连环蛋白丢失。在大多数脑膜瘤中检测到β-连环蛋白的核周颗粒免疫反应强烈,无核定位。分离的脑膜瘤细胞免疫荧光和westernblot分析显示β-catenin主要位于高尔基体和ER/Golgi中间区(ERGIC)。脑膜瘤的细胞遗传学分析显示NF2丢失与β-连环蛋白正确定位丢失之间没有相关性。

CONCLUSIONS

脑膜瘤中缺乏膜β-连环蛋白和/或膜E-钙粘蛋白可能表明脑膜瘤细胞之间相互作用的改变,与NF2的丢失无关,与肿瘤分级无关。


AIMS

Meningiomas are generally slow-growing benign tumours representing approximately 20% of all primary intracranial tumours. The hallmark of tumorigenesis of meningiomas is the loss of chromosome 22, including loss of heterozygosity of the neurofibromatosis type 2 (NF2) gene. The NF2 encoded protein merlin appears to function as a tumour suppressor gene by controlling cadherin-mediated cell-cell adhesion. The E-cadherin cell adhesion system includes beta-catenin that indirectly connects cadherin to actin filaments. The aim of this study was to analyse the expression and the subcellular location of E-cadherin and beta-catenin in human meningiomas, including meningiomas of different histomorphological subtypes and different World Health Organization (WHO) grades.

METHODS AND RESULTS

Immunohistochemical analysis revealed lack of E-cadherin expression at the cell membrane in 34% of meningiomas independent of their WHO grade. Loss of membranous beta-catenin occurred in 79% of meningiomas. An intense perinuclear granular immunoreactivity of beta-catenin without nuclear location was detected in the majority of meningiomas. Both immunofluorescence and Western blot analysis of fractionated meningioma cells located beta-catenin mostly on the Golgi apparatus and ER/Golgi intermediate compartment (ERGIC). Cytogenetic analysis of meningiomas showed no correlation between NF2 loss and the loss of the proper location of beta-catenin.

CONCLUSIONS

The lack of membranous beta-catenin and/or membranous E-cadherin in meningiomas may indicate an altered interaction between meningioma cells independent of loss of NF2 and independent of the tumour grade.


获取全文 10.1111/j.1365-2559.2006.02440.x