[水蛭与虻合用对血瘀证大鼠模型的抗凝和抗血小板聚集作用]。

PubMed ID
发表日期 2009年Sep月

原始出处 钟耀才
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
作者 Liang  Jin-quan  Mi  Sui-qing  Wang  Ning-sheng 

文献标题 [水蛭与虻合用对血瘀证大鼠模型的抗凝和抗血小板聚集作用]。
[Anticoagulative effect and antiplatelet aggregation effect of combination of Hirudo and Tabanus on rat model of blood stasis syndrome].

文献摘要 OBJECTIVE

目的观察水蛭与不同剂量比虻合用对血瘀证大鼠模型的抗凝和抗血小板聚集作用。

METHODS

采用皮下注射肾上腺素结合冰水刺激建立血瘀证大鼠模型。测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)含量及血小板聚集抑制率。

RESULTS

模型对照组血小板聚集增加,APTT、PT降低,FIB含量明显升高(P<0.001)。水蛭、烟粉虱及水蛭与烟粉虱配伍均不同程度地具有抗血小板聚集作用。水蛭组、虻组和合用组APTT和PT均显著延长(P<0.05和P<0.01),水蛭与虻剂量比为4:3组更为明显。联合用药组FIB含量明显降低,剂量比为3:1(P<0.05)。

CONCLUSIONS

水蛭与虻合用组的抗凝和抗血小板聚集作用优于水蛭组和虻合用组。而在四个联合用药组中,经典中医专著推荐的水蛭与虻的剂量比为4:3的组抗凝效果最好。


OBJECTIVE

To observe anticoagulative effect and antiplatelet aggregation effect of the combination of Hirudo and Tabanus with different dose-ratio on rat model of blood stasis syndrome.

METHODS

The rat model of blood stasis syndrome was established by subcutaneous injection of adrenaline combined with stimulation of icy water. Then prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) contents and inhibition rate of blood platelet aggregation were determined.

RESULTS

Platelet aggregation increases, APTT and PT reduced, and FIB contents increased in model control group significantly (P<0.001). Hirudo, Tabanus and the combination of Hirudo and Tabanus had antiplatelet aggregation effect in varying degrees. APTT and PT were prolonged significantly (P<0.05 and P<0.01, respectively) in Hirudo group, Tabanus group and combination groups, especially in the group with dose-ratio of Hirudo to Tabanus being 4:3. FIB contents decreased significantly in combination group with dose-ratio being 3:1 (P<0.05).

CONCLUSIONS

The combination groups of Hirudo and Tabanus have better effect of anticoagulation and antiplatelet aggregation than Hirudo group and Tabanus group. While in the four combination groups, the group recommended by classical TCM monograph with dose-ratio of Hirudo to Tabanus being 4:3, has the best anticoagulation effect.