杜仲的降压作用。自发性高血压大鼠的提取物。

PubMed ID
G H
发表日期 2010年May月

原始出处 民族药理学杂志
Journal of ethnopharmacology
作者 Luo  Li-fang  Wu  Wei-hua  Zhou  Ying-jun  Yan  Jin  Yang  Guo-ping  Ouyang  Dong-sheng 

文献标题 杜仲的降压作用。自发性高血压大鼠的提取物。
Antihypertensive effect of Eucommia ulmoides Oliv. extracts in spontaneously hypertensive rats.
Antihypertensive effect of Eucommia ulmoides Oliv. extracts in spontaneously hypertensive rats.

文献摘要 AIM OF THE STUDY

目的研究杜仲降压部位。及其在自发性高血压大鼠(shr)中的作用机制。

MATERIALS AND METHODS

在体实验中,给SD大鼠和shr灌胃杜仲木脂素(EuL)和杜仲环烯醚萜(EuI),测定其血压。采用比色法测定血浆一氧化氮(NO)水平,放射免疫法测定肾素活性(RA)和血管紧张素Ⅱ(AngⅡ)浓度。在体外实验中,用EuL处理大鼠肠系膜动脉,测定血管舒张反应。

RESULTS

EuL可剂量依赖性地降低SD大鼠和SHR大鼠的血压,但EuL对两种大鼠血压无影响。同时,EuL和EuI的联合作用没有观察到协同效应。每日2次eul300mg/kg可显著提高SHR血浆NO水平。长期口服eul150和300mg/kg,每日2次,血浆RA和AngⅡ水平均下降。在灌流实验中,EuL快速、剂量依赖性地舒张肠系膜动脉,对有内皮和无内皮动脉的影响相同。

CONCLUSIONS

EuL可能是降压的有效部位,其降压作用可能与调节NO和肾素血管紧张素系统(RAS)及直接舒张动脉有关。


AIM OF THE STUDY

To investigate the antihypertensive fractions of Eucommia ulmoides Oliv. and their underlying mechanisms in spontaneously hypertensive rats (SHRs).

MATERIALS AND METHODS

In vivo study, Eucommia ulmoides lignans (EuL) and Eucommia ulmoides iridoids (EuI) were administrated to Sprague-Dawley (SD) rats and SHRs, and their blood pressures were measured. Plasma level of nitric oxide (NO) was measured by colorimetric method, and renin activity (RA) and plasma concentration of angiotensin II (Ang II) were measured by radioimmunoassay. In vitro study, rat mesenteric artery was treated with EuL and the vessel relaxation responses were determined.

RESULTS

EuL could lower blood pressures of both SD rats and SHR dose-dependently by either intravenous (i.v.) or intragastric (i.g.) administration, but EuI failed to affect blood pressure in the two kinds of rats. Meanwhile, no synergistic effect was observed with the combination of EuL and EuI. The plasma level of NO in SHR treated with EuL 300 mg/kg twice a day was markedly increased. Both plasma RA and Ang II level were decreased with long-term oral treatment of EuL 150 and 300 mg/kg twice a day. In perfusion experiment, EuL relaxed mesenteric artery quickly and dose-dependently and the effect on the artery with and without endothelium was the same.

CONCLUSIONS

EuL may be the effective fraction to lowering blood pressure and its antihypertensive effect is probably associated with regulating NO and renin-angiotensin system (RAS) and directly relaxing artery.

AIM OF THE STUDY

To investigate the antihypertensive fractions of Eucommia ulmoides Oliv. and their underlying mechanisms in spontaneously hypertensive rats (SHRs).

MATERIALS AND METHODS

In vivo study, Eucommia ulmoides lignans (EuL) and Eucommia ulmoides iridoids (EuI) were administrated to Sprague-Dawley (SD) rats and SHRs, and their blood pressures were measured. Plasma level of nitric oxide (NO) was measured by colorimetric method, and renin activity (RA) and plasma concentration of angiotensin II (Ang II) were measured by radioimmunoassay. In vitro study, rat mesenteric artery was treated with EuL and the vessel relaxation responses were determined.

RESULTS

EuL could lower blood pressures of both SD rats and SHR dose-dependently by either intravenous (i.v.) or intragastric (i.g.) administration, but EuI failed to affect blood pressure in the two kinds of rats. Meanwhile, no synergistic effect was observed with the combination of EuL and EuI. The plasma level of NO in SHR treated with EuL 300 mg/kg twice a day was markedly increased. Both plasma RA and Ang II level were decreased with long-term oral treatment of EuL 150 and 300 mg/kg twice a day. In perfusion experiment, EuL relaxed mesenteric artery quickly and dose-dependently and the effect on the artery with and without endothelium was the same.

CONCLUSIONS

EuL may be the effective fraction to lowering blood pressure and its antihypertensive effect is probably associated with regulating NO and renin-angiotensin system (RAS) and directly relaxing artery.


获取全文 10.1016/j.jep.2010.03.019