稀硫酸预处理甘蔗渣样品的研究。

PubMed ID
发表日期 2011年Sep月

原始出处 工业微生物学与生物技术杂志
Journal of industrial microbiology & biotechnology
作者 Canilha  Larissa  Santos  Victor T O  Rocha  George J M  Almeida e Silva  João B  Giulietti  Marco  Silva  Silvio S  Felipe  Maria G A  Ferraz  André  Milagres  Adriane M F  Carvalho  Walter 

文献标题 稀硫酸预处理甘蔗渣样品的研究。
A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid.

文献摘要

采用2(3)中心复合全析因设计,在200ml不锈钢容器中进行了甘蔗渣样品的稀硫酸预处理试验。研究的自变量为温度,变化范围为112.5°C至157.5°C、 停留时间为5.0~35.0min,硫酸浓度为0.0%~3.0%(w/v)。所有试验均采用15%(w/w)的蔗渣负载量。对实验结果的统计分析表明,这三个自变量对响应变量都有显著的影响,即蔗渣的增溶率、半纤维素水解液中木糖的回收率、纤维素酶糖化效率以及纤维素、半纤维素、纤维素和纤维素的百分比,以及预处理固体中的木质素。温度对响应变量的影响最大,其次是酸浓度和停留时间。尽管较苛刻的预处理条件促进了半纤维素部分的几乎完全去除,但半纤维素水解产物中木糖的回收量不超过最大理论值的61.8%。纤维素酶糖化有利于在预处理过程中更有效地去除半纤维素。然而,半纤维素水解物的脱毒对于糖类更好的生物转化为乙醇是必要的。


Experiments based on a 2(3) central composite full factorial design were carried out in 200-ml stainless-steel containers to study the pretreatment, with dilute sulfuric acid, of a sugarcane bagasse sample obtained from a local sugar-alcohol mill. The independent variables selected for study were temperature, varied from 112.5°C to 157.5°C, residence time, varied from 5.0 to 35.0 min, and sulfuric acid concentration, varied from 0.0% to 3.0% (w/v). Bagasse loading of 15% (w/w) was used in all experiments. Statistical analysis of the experimental results showed that all three independent variables significantly influenced the response variables, namely the bagasse solubilization, efficiency of xylose recovery in the hemicellulosic hydrolysate, efficiency of cellulose enzymatic saccharification, and percentages of cellulose, hemicellulose, and lignin in the pretreated solids. Temperature was the factor that influenced the response variables the most, followed by acid concentration and residence time, in that order. Although harsher pretreatment conditions promoted almost complete removal of the hemicellulosic fraction, the amount of xylose recovered in the hemicellulosic hydrolysate did not exceed 61.8% of the maximum theoretical value. Cellulose enzymatic saccharification was favored by more efficient removal of hemicellulose during the pretreatment. However, detoxification of the hemicellulosic hydrolysate was necessary for better bioconversion of the sugars to ethanol.


获取全文 10.1007/s10295-010-0931-2