以脂肪生成减少和脂质代谢调节为特征的麦冬的减肥作用。

PubMed ID
发表日期 2011年Apr月

原始出处 实验与分子医学
Experimental & molecular medicine
作者 Seo  Jong Bae  Choe  Sung Sik  Jeong  Hyun Woo  Park  Sang Wook  Shin  Hyun Jung  Choi  Sun Mi  Park  Jae Young  Choi  Eun Wook  Kim  Jae Bum  Seen  Dong Seung  Jeong  Jae-Yeon  Lee  Tae Gyu 

文献标题 以脂肪生成减少和脂质代谢调节为特征的麦冬的减肥作用。
Anti-obesity effects of Lysimachia foenum-graecum characterized by decreased adipogenesis and regulated lipid metabolism.

文献摘要

麦冬是一种具有抗炎作用的东方药。在筛选天然产物提取物文库中首次发现了罗汉果提取物(LFE)的减肥作用。为了研究其减肥作用并评价其作为减肥药物的潜力,我们在体内外进行了各种与肥胖相关的实验。在成脂实验中,LFE以2.5μg/ml的IC50抑制3T3-L1前脂肪细胞的分化,并呈剂量依赖性。此外,LFE抑制成脂基因的表达,同时在10μg/ml的体外和100mg/kg/d的体内增加成脂基因的表达。LFE的抗脂肪和抗脂作用可能是通过抑制PPARγ和C/EBPα在体内外的表达,以及抑制PPARγ在体外的活性来介导的。此外,LFE以AMPK依赖的方式刺激脂肪酸氧化。在高脂饮食(HFD)诱导的肥胖小鼠(n=8/组)中,30、100和300 mg/kg/天口服LFE显著降低了所有试验剂量的总体重增加。两组小鼠的食物摄取量没有差异。LFE处理的HFD小鼠腹部皮下、附睾和肾周脂肪组织重量明显减轻,呈剂量依赖性。治疗LFE也大大提高了肥胖相关生物标志物的血清水平,如葡萄糖、甘油三酯和脂肪细胞因子瘦素、脂联素和抵抗素。综上所述,LFE在体内外对脂肪生成和脂质代谢有一定的抑制作用,为LFE作为减肥药物的开发提供了可能。


Lysimachia foenum-graecum has been used as an oriental medicine with anti-inflammatory effect. The anti-obesity effect of L. foenum-graecum extract (LFE) was first discovered in our screening of natural product extract library against adipogenesis. To characterize its anti-obesity effects and to evaluate its potential as an anti-obesity drug, we performed various obesity-related experiments in vitro and in vivo. In adipogenesis assay, LFE blocked the differentiation of 3T3-L1 preadipocyte in a dose-dependent manner with an IC50 of 2.5 μg/ml. In addition, LFE suppressed the expression of lipogenic genes, while increasing the expression of lipolytic genes in vitro at 10 μg/ml and in vivo at 100 mg/kg/day. The anti-adipogenic and anti-lipogenic effect of LFE seems to be mediated by the inhibition of PPARγ and C/EBPα expression as shown in in vitro and in vivo, and the suppression of PPARγ activity in vitro. Moreover, LFE stimulated fatty acid oxidation in an AMPK-dependent manner. In high-fat diet (HFD)-induced obese mice (n = 8/group), oral administration of LFE at 30, 100, and 300 mg/kg/day decreased total body weight gain significantly in all doses tested. No difference in food intake was observed between vehicle- and LFE-treated HFD mice. The weight of white adipose tissues including abdominal subcutaneous, epididymal, and perirenal adipose tissue was reduced markedly in LFE-treated HFD mice in a dose-dependent manner. Treatment of LFE also greatly improved serum levels of obesity-related biomarkers such as glucose, triglycerides, and adipocytokines leptin, adiponectin, and resistin. All together, these results showed anti-obesity effects of LFE on adipogenesis and lipid metabolism in vitro and in vivo and raised a possibility of developing LFE as anti-obesity therapeutics.


获取全文 10.3858/emm.2011.43.4.025