杜仲的抑菌作用。东莨菪碱对小鼠学习记忆障碍的影响。

PubMed ID
G H
发表日期 2013年Nov月

原始出处 生物分子与治疗学
Biomolecules & therapeutics
作者 Kwon  Seung-Hwan  Ma  Shi-Xun  Joo  Hyun-Joong  Lee  Seok-Yong  Jang  Choon-Gon 

文献标题 杜仲的抑菌作用。东莨菪碱对小鼠学习记忆障碍的影响。
Inhibitory Effects of Eucommia ulmoides Oliv. Bark on Scopolamine-Induced Learning and Memory Deficits in Mice.
Inhibitory Effects of Eucommia ulmoides Oliv. Bark on Scopolamine-Induced Learning and Memory Deficits in Mice.

文献摘要

杜仲。树皮(EUE)通常用于治疗高血压、类风湿性关节炎、腰痛和坐骨痛以及促进长寿。在这项研究中,我们测试了不同剂量的EUE水提物对东莨菪碱诱导的小鼠学习记忆障碍的保护和增强认知的作用。通过被动回避和Morris水迷宫实验,EUE能显著改善东莨菪碱对小鼠Y迷宫短期记忆和工作记忆的损伤,并能显著逆转小鼠的学习记忆障碍。最后一次Morris水迷宫试验(探针试验)后一天,EUE以剂量依赖性方式显著增加了靶区的潜伏期。此外,EUE显著抑制海马和额叶皮层乙酰胆碱酯酶(AChE)和硫代巴比妥酸反应物质(TBARS)活性,且呈剂量依赖性。EUE还显著增加了东莨菪碱诱导的小鼠海马脑源性神经营养因子(BDNF)和cAMP结合蛋白(CREB)的磷酸化。基于这些发现,我们认为EUE可能有助于治疗认知功能障碍,并且EUE的有益作用部分是通过胆碱能信号增强和/或保护介导的。


Eucommia ulmoides Oliv. Bark (EUE) is commonly used for the treatment of hypertension, rheumatoid arthritis, lumbago, and ischialgia as well as to promote longevity. In this study, we tested the effects of EUE aqueous extract in graded doses to protect and enhance cognition in scopolamine-induced learning and memory impairments in mice. EUE significantly improved the impairment of short-term or working memory induced by scopolamine in the Y-maze and significantly reversed learning and memory deficits in mice as measured by the passive avoidance and Morris water maze tests. One day after the last trial session of the Morris water maze test (probe trial session), EUE dramatically increased the latency time in the target quadrant in a dose-dependent manner. Furthermore, EUE significantly inhibited acetylcholinesterase (AChE) and thiobarbituric acid reactive substance (TBARS) activities in the hippocampus and frontal cortex in a dose-dependent manner. EUE also markedly increased brain-derived neurotrophic factor (BDNF) and phosphorylation of cAMP element binding protein (CREB) in the hippocampus of scopolamine-induced mice. Based on these findings, we suggest that EUE may be useful for the treatment of cognitive deficits, and that the beneficial effects of EUE are mediated, in part, by cholinergic signaling enhancement and/or protection.

Eucommia ulmoides Oliv. Bark (EUE) is commonly used for the treatment of hypertension, rheumatoid arthritis, lumbago, and ischialgia as well as to promote longevity. In this study, we tested the effects of EUE aqueous extract in graded doses to protect and enhance cognition in scopolamine-induced learning and memory impairments in mice. EUE significantly improved the impairment of short-term or working memory induced by scopolamine in the Y-maze and significantly reversed learning and memory deficits in mice as measured by the passive avoidance and Morris water maze tests. One day after the last trial session of the Morris water maze test (probe trial session), EUE dramatically increased the latency time in the target quadrant in a dose-dependent manner. Furthermore, EUE significantly inhibited acetylcholinesterase (AChE) and thiobarbituric acid reactive substance (TBARS) activities in the hippocampus and frontal cortex in a dose-dependent manner. EUE also markedly increased brain-derived neurotrophic factor (BDNF) and phosphorylation of cAMP element binding protein (CREB) in the hippocampus of scopolamine-induced mice. Based on these findings, we suggest that EUE may be useful for the treatment of cognitive deficits, and that the beneficial effects of EUE are mediated, in part, by cholinergic signaling enhancement and/or protection.


获取全文 10.4062/biomolther.2013.074