密切相关的CD103+树突状细胞(DCs)和淋巴驻留的CD8+DCs在炎症功能上存在差异。

PubMed ID
发表日期 2014年月

原始出处 公共科学图书馆一号
PloS one
作者 Jiao  Zhijun  Bedoui  Sammy  Brady  Jamie L  Walter  Anne  Chopin  Michael  Carrington  Emma M  Sutherland  Robyn M  Nutt  Stephen L  Zhang  Yuxia  Ko  Hyun-Ja  Wu  Li  Lew  Andrew M  Zhan  Yifan 

文献标题 密切相关的CD103+树突状细胞(DCs)和淋巴驻留的CD8+DCs在炎症功能上存在差异。
The closely related CD103+ dendritic cells (DCs) and lymphoid-resident CD8+ DCs differ in their inflammatory functions.

文献摘要

移行性CD103+和淋巴驻留性CD8+树突状细胞(dc)具有许多共同的特性,如对同一转录因子的依赖性、交叉呈递能力和某些表面分子的表达,因此有人认为它们属于一个共同的亚系。然而,这两种DC类型的功能多样性尚不完全清楚。我们发现,当皮肤感染单纯疱疹病毒时,从引流淋巴结移行的CD103+dc比CD8+dc更能诱导CD4+T细胞产生Th17细胞因子。在未感染小鼠的CD103+dc中,这种驱动Th17反应的优越能力也很明显。与CD8+DCs相比,CD103+DCs产生的IL-1β和IL-6更高,反映了它们诱导Th17分化的不同潜能。分离淋巴结的两种dc在某些模式识别受体的表达上也不同。此外,GM-CSF水平升高(典型的炎症反应)显著增加了淋巴结和皮肤中CD103+DC的数量。我们认为不同水平的GM-CSF可以解释关于GM-CSF在调节CD103+DC发育中的积极作用的对比报道。总之,我们发现这两个发展密切相关的DC亚群显示功能差异,GM-CSF对这两种类型的DC有不同的作用。


Migratory CD103+ and lymphoid-resident CD8+ dendritic cells (DCs) share many attributes, such as dependence on the same transcription factors, cross-presenting ability and expression of certain surface molecules, such that it has been proposed they belong to a common sub-lineage. The functional diversity of the two DC types is nevertheless incompletely understood. Here we reveal that upon skin infection with herpes simplex virus, migratory CD103+ DCs from draining lymph nodes were more potent at inducing Th17 cytokine production by CD4+ T cells than CD8+ DCs. This superior capacity to drive Th17 responses was also evident in CD103+ DCs from uninfected mice. Their differential potency to induce Th17 differentiation was reflected by higher production of IL-1β and IL-6 by CD103+ DCs compared with CD8+ DCs upon stimulation. The two types of DCs from isolated lymph nodes also differ in expression of certain pattern recognition receptors. Furthermore, elevated levels of GM-CSF, typical of those found in inflammation, substantially increased the pool size of CD103+ DCs in lymph nodes and skin. We argue that varied levels of GM-CSF may explain the contrasting reports regarding the positive role of GM-CSF in regulating development of CD103+ DCs. Together, we find that these two developmentally closely-related DC subsets display functional differences and that GM-CSF has differential effect on the two types of DCs.


获取全文 10.1371/journal.pone.0091126