表面化学灭活HIV。

PubMed ID
发表日期 1989年Apr月

原始出处 BMJ(临床研究版)
BMJ (Clinical research ed.)
作者 Hanson  P J  Gor  D  Jeffries  D J  Collins  J V 

文献标题 表面化学灭活HIV。
Chemical inactivation of HIV on surfaces.

文献摘要

为了评估酒精和戊二醛是否对干燥的HIV有效消毒剂,测试了70%酒精(乙醇和工业甲基化酒精)以及1%和2%碱性戊二醛对干燥在表面的细胞相关和无细胞HIV的杀灭效果。将病毒储备(100微升)或10000个培养的感染HIV的C8166 T淋巴细胞干燥在无菌盖玻片上,并在2%和1%碱性戊二醛和70%乙醇中浸泡30秒、1、2、4和10分钟,用70%的工业甲基化酒精消毒无细胞病毒有20分钟的额外时间点。另外,用1%和2%碱性戊二醛检测纯血清中的病毒储备,看戊二醛的固定性能是否损害其杀毒性能。消毒后的病毒活性通过将盖玻片(细胞相关病毒)或盖玻片和超声无细胞病毒与C8166 T淋巴细胞孵育来检测。检测淋巴细胞是否形成合胞体,并在培养液中检测HIV抗原。2%和1%碱性戊二醛均能在1min内灭活无细胞HIV;2%碱性戊二醛也能在2min内灭活血清中的无细胞病毒,但1%的溶液浸泡15min无效。2%碱性戊二醛在2分钟内灭活细胞相关的HIV。70%的工业甲基化酒精未能在20分钟和15分钟内灭活无细胞和与细胞相关的艾滋病毒,70%的乙醇在10分钟内没有灭活无细胞病毒。70%的工业甲基化酒精和乙醇不适用于HIV的表面消毒。新鲜的2%碱性戊二醛溶液是有效的,但当用于对与有机物有关的HIV进行消毒时,应注意不要太稀或没有变质。


To assess whether alcohol and glutaraldehyde are effective disinfectants against dried HIV the virucidal effects of 70% alcohol (ethanol and industrial methylated spirit) and 1% and 2% alkaline glutaraldehyde were tested against cell associated and cell free HIV dried on to a surface. Virus stock (100 microliters) or 10,000 cultured C8166 T lymphocytes infected with HIV were dried onto sterile coverslips and immersed in 2% and 1% alkaline glutaraldehyde and 70% ethanol for 30 seconds and one, two, four, and 10 minutes, there being an additional time point of 20 minutes for cell free virus disinfected with 70% industrial methylated spirit. In addition, virus stock in neat serum was tested with 1% and 2% alkaline glutaraldehyde to see whether the fixative properties of glutaraldehyde impair its virucidal properties. Virus activity after disinfection was tested by incubating the coverslips (cell associated virus) or the coverslips and sonicated cell free virus with C8166 T lymphocytes. The lymphocytes were examined for the formation of syncytia and HIV antigens were assayed in the culture fluid. Both 2% and 1% alkaline glutaraldehyde inactivated cell free HIV within one minute; 2% alkaline glutaraldehyde also inactivated cell free virus in serum within two minutes, but a 1% solution was ineffective after 15 minutes' immersion. Cell associated HIV was inactivated by 2% alkaline glutaraldehyde within two minutes. Seventy per cent industrial methylated spirit failed to inactivate cell free and cell associated HIV within 20 and 15 minutes, respectively, and 70% ethanol did not inactivate cell free virus within 10 minutes. Seventy per cent industrial methylated spirit and ethanol are not suitable for surface disinfection of HIV. Fresh 2% solutions of alkaline glutaraldehyde are effective, but care should be taken that they are not too dilute or have not become stale when used for disinfecting HIV associated with organic matter.


获取全文 10.1136/bmj.298.6677.862