杜仲树脂纯化工艺优化及降压作用机理研究。

PubMed ID
G H
发表日期 2014年月

原始出处 非洲传统、补充和替代药物杂志:AJTCAM
African journal of traditional, complementary, and alternative medicines : AJTCAM
作者 Yongsheng  Liu  Shumei  Li  Guodong  Wu 

文献标题 杜仲树脂纯化工艺优化及降压作用机理研究。
Studies on resin purification process optimization of Eucommia ulmoides Oliver and its antihypertensive effect mechanism.
Studies on resin purification process optimization of Eucommia ulmoides Oliver and its antihypertensive effect mechanism.

文献摘要 BACKGROUND

通过研究发现,杜仲皮、茎、果、花中含有木质素、环烷烃、苯丙烷等多种成分。现代药理研究表明,杜仲具有抗衰老、抗肿瘤、促进骨细胞增殖等作用。研究了杜仲树脂纯化工艺的优化条件及降压作用。

MATERIALS AND METHODS

采用正交试验法考察了各种大孔树脂对杜仲的纯化效果,并采用SHR模型测定了杜仲的降压水平。

RESULTS

以20%乙醇为洗脱剂,负载液浓度为6mg/ml,流速控制为3.0,柱体积/h,吸附柱径高比为1:6,确定了杜仲树脂提取纯化的最佳工艺条件SHR降压效果相对稳定,对HR无明显影响,但降压效果低于对照药物卡托普利。杜仲提取物能显著提高血清NO含量,降低ET含量。

CONCLUSION

杜仲能松弛血管,降低外周阻力,减少返血量,最终达到降压效果。


BACKGROUND

Through research it is found that the barks, stems, fruits and flowers of Eucommia ulmoides contain various compositions, such as lignin, cycloalkane and phenylpropanoids. The modern pharmacological research shows that Eucommia ulmoides has many functions, such as anti-aging, antitumor and bone cell proliferation. This paper studied the resin purification process optimization conditions and antihypertensive effect of Eucommia ulmoides Oliver.

MATERIALS AND METHODS

The orthogonal experiment method was adopted to determine the purification effect of various macro-porous resins for eucommia ulmoides Oliver, and the SHR model was used to determine antihypertensive level of eucommia ulmoides Oliver.

RESULTS

The optimal process of resin extraction and purification of Eucommia ulmoides Oliver was verified through the loading liquid concentration at 6 mg/ml, 20% ethanol used as the eluent; the flow rate control was 3.0, column volume per hour and the diameter-height ratio of adsorption column was 1:6; the extracts of Eucommia ulmoides Oliver can reduce SHR blood pressure, its antihypertensive effect is relatively stable and has no significant impact on HR, but the antihypertensive effect is lower than that of control medicine captopril. Also, the extracts of eucommia ulmoides Oliver can significantly increase the content of serum NO and reduce the content of ET.

CONCLUSION

The Eucommia ulmoides Oliver can relax blood vessels, reduce the peripheral resistance, reduce the returned blood volume, and eventually achieve the antihypertensive effect.

BACKGROUND

Through research it is found that the barks, stems, fruits and flowers of Eucommia ulmoides contain various compositions, such as lignin, cycloalkane and phenylpropanoids. The modern pharmacological research shows that Eucommia ulmoides has many functions, such as anti-aging, antitumor and bone cell proliferation. This paper studied the resin purification process optimization conditions and antihypertensive effect of Eucommia ulmoides Oliver.

MATERIALS AND METHODS

The orthogonal experiment method was adopted to determine the purification effect of various macro-porous resins for eucommia ulmoides Oliver, and the SHR model was used to determine antihypertensive level of eucommia ulmoides Oliver.

RESULTS

The optimal process of resin extraction and purification of Eucommia ulmoides Oliver was verified through the loading liquid concentration at 6 mg/ml, 20% ethanol used as the eluent; the flow rate control was 3.0, column volume per hour and the diameter-height ratio of adsorption column was 1:6; the extracts of Eucommia ulmoides Oliver can reduce SHR blood pressure, its antihypertensive effect is relatively stable and has no significant impact on HR, but the antihypertensive effect is lower than that of control medicine captopril. Also, the extracts of eucommia ulmoides Oliver can significantly increase the content of serum NO and reduce the content of ET.

CONCLUSION

The Eucommia ulmoides Oliver can relax blood vessels, reduce the peripheral resistance, reduce the returned blood volume, and eventually achieve the antihypertensive effect.


获取全文 10.4314/ajtcam.v11i2.36