红景天提取物通过激活AMPK途径抑制肝糖异生。

PubMed ID
发表日期 2015年Apr月

原始出处 植物医学:国际植物治疗和植物药理学杂志
Phytomedicine : international journal of phytotherapy and phytopharmacology
作者 Lee  Shih-Yu  Lai  Feng-Yi  Shi  Li-Shian  Chou  Yu-Ching  Yen  I-Chuan  Chang  Tsu-Chung 

文献标题 红景天提取物通过激活AMPK途径抑制肝糖异生。
Rhodiola crenulata extract suppresses hepatic gluconeogenesis via activation of the AMPK pathway.

文献摘要 BACKGROUND

红景天是一种很受欢迎的草本植物,被用来治疗高原疾病、抑郁、疲劳和糖尿病。然而,红景天在肝脏中发挥作用的详细机制需要进一步阐明。

PURPOSE

本研究旨在探讨红景天根提取物(RCE)对肝脏葡萄糖生成的影响。

METHODS

用RCE处理人肝癌HepG2细胞6h,测定其产糖量、p-AMPK的表达水平及糖异生关键基因的表达。研究了RCE对SD大鼠的影响。探讨了RCE在肝脏中的作用机制。

RESULTS

RCE在激活AMPK信号通路的同时,显著抑制HepG2细胞的葡萄糖生成和糖异生基因的表达。有趣的是,RCE抑制的肝糖异生作用被AMPK特异性抑制剂消除,而PI3K/AKT特异性抑制剂不能消除。此外,口服RCE可显著提高大鼠肝脏磷酸化AMPK水平,抑制糖异生基因表达。此外,RCE治疗还降低了大鼠的血糖浓度。

CONCLUSION

体外和体内实验证明,RCE可能通过激活AMPK信号通路抑制肝脏糖异生而发挥降糖作用。这些发现为红景天可能有助于II型糖尿病的治疗提供了证据。


BACKGROUND

Rhodiola, a popular herb, has been used for treating high altitude sicknesses, depression, fatigue, and diabetes. However, the detailed mechanisms by which Rhodiola crenulata functions in the liver need further clarification.

PURPOSE

The current study was designed to examine the effects of Rhodiola crenulata root extract (RCE) on hepatic glucose production.

METHODS

Human hepatoma HepG2 cells were treated with RCE for 6 h. Glucose production, the expression level of p-AMPK, and the expression of key gluconeogenic genes were measured. The effects of RCE were also studied in Sprague-Dawley (SD) rats. The efficacy and underlying mechanism of RCE in the liver were examined.

RESULTS

RCE significantly suppressed glucose production and gluconeogenic gene expression in HepG2 cells while activating the AMPK signaling pathway. Interestingly, RCE-suppressed hepatic gluconeogenesis was eliminated by an AMPK-specific inhibitor, but not by the PI3K/AKT-specific inhibitor. In addition, oral administration of RCE significantly increased phosphorylated AMPK levels and inhibited gluconeogenic gene expression in the rat liver. Furthermore, RCE treatment also decreased plasma glucose concentration in rats.

CONCLUSION

We present in vitro and in vivo evidence that RCE might exert the glucose-lowering effect partly by inhibiting hepatic gluconeogenesis through activating the AMPK signaling pathway. These findings provide evidence that Rhodiola crenulata may be helpful for the management of type II diabetes.


获取全文 10.1016/j.phymed.2015.01.016