钩藤有效成分与莱菔子合用对N’-硝基-L-精氨酸(L-NNA)诱导的高血压大鼠血管内皮细胞的保护作用。

PubMed ID
G H
发表日期 2015年Aug月

原始出处 生物科学趋势
Bioscience trends
作者 Li  Yunlun  Yang  Wenqing  Zhu  Qingjun  Yang  Jinguo  Wang  Zhen 

文献标题 钩藤有效成分与莱菔子合用对N’-硝基-L-精氨酸(L-NNA)诱导的高血压大鼠血管内皮细胞的保护作用。
Protective effects on vascular endothelial cell in N'-nitro-L-arginine (L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani.
Protective effects on vascular endothelial cell in N'-nitro-L-arginine (L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani.

文献摘要

内皮功能障碍与高血压密切相关。保护血管内皮细胞是防治高血压的关键。钩藤总生物碱和莱菔子可溶性生物碱分别从中药钩藤和莱菔子中提取,具有抗高血压和保护血管的作用。本文观察钩藤总生物碱和莱菔子可溶性生物碱联合应用对N’-硝基-L-精氨酸诱导的高血压大鼠血管内皮细胞的保护作用,并初步探讨其作用机制。采用无创大鼠尾法测定血压,观察药物的降压作用。扫描电镜观察血管内皮细胞的完整性或脱落状态。检测循环内皮细胞数量及循环内皮细胞CD54、CD62P表达,评价内皮功能。本研究发现钩藤总生物碱与莱菔子水溶性生物碱配伍能有效降低血压,改善血管内皮结构完整性,显著减少循环内皮细胞数量。钩藤总生物碱和莱菔子可溶性生物碱配伍干预后,CD54和CD62P表达的平均荧光强度降低。综上所述,钩藤总生物碱和莱菔子水溶性生物碱有效成分联合应用具有良好的降压作用和血管内皮保护作用。其保护作用的初步机制可能归因于减轻全身低度炎症。


Endothelial dysfunction is closely associated with hypertension. Protection of vascular endothelial cell is the key to prevention and treatment of hypertension. Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid, isolated from traditional Chinese medicine Uncaria rbyncbopbylla and Semen Raphani respectively, exhibit properties of anti-hypertension and protection of blood vessels. In the present study, we observed the protective effect of the combined use of Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid to the vascular endothelial cell in N'-nitro-L-arginine-induced hypertensive rats and investigate the preliminary mechanism. Blood pressure was detected by non-invasive rats tail method to observe the anti-hypertension effect of drugs. Scanning electron microscopy was used to observe the integrity or shedding state of vascular endothelial cell. The amount of circulating endothelial cells and CD54 and CD62P expression on circulating endothelial cells were tested to evaluate the endothelium function. In this study, we found that the Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid compatibility can effectively lower the blood pressure, improve the structural integrity of vascular endothelium, and significantly reduce the number of circulating endothelial cells. Furthermore, the mean fluorescence intensity of CD54 and CD62P expressed showed decrease after the intervention of Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid compatibility. In conclusion, the combination of effective components of the Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid demonstrated good antihypertension effect and vascular endothelium protective effect. The preliminary mechanism of the protective effect may attribute to relieve the overall low-grade inflammation.

Endothelial dysfunction is closely associated with hypertension. Protection of vascular endothelial cell is the key to prevention and treatment of hypertension. Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid, isolated from traditional Chinese medicine Uncaria rbyncbopbylla and Semen Raphani respectively, exhibit properties of anti-hypertension and protection of blood vessels. In the present study, we observed the protective effect of the combined use of Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid to the vascular endothelial cell in N'-nitro-L-arginine-induced hypertensive rats and investigate the preliminary mechanism. Blood pressure was detected by non-invasive rats tail method to observe the anti-hypertension effect of drugs. Scanning electron microscopy was used to observe the integrity or shedding state of vascular endothelial cell. The amount of circulating endothelial cells and CD54 and CD62P expression on circulating endothelial cells were tested to evaluate the endothelium function. In this study, we found that the Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid compatibility can effectively lower the blood pressure, improve the structural integrity of vascular endothelium, and significantly reduce the number of circulating endothelial cells. Furthermore, the mean fluorescence intensity of CD54 and CD62P expressed showed decrease after the intervention of Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid compatibility. In conclusion, the combination of effective components of the Uncaria rhynchophylla total alkaloids and Semen Raphani soluble alkaloid demonstrated good antihypertension effect and vascular endothelium protective effect. The preliminary mechanism of the protective effect may attribute to relieve the overall low-grade inflammation.


获取全文 10.5582/bst.2015.01087