钩藤对LPS刺激的BV2小胶质细胞和局灶性脑缺血小鼠的抗炎作用。

PubMed ID
G H
发表日期 2015年月

原始出处 美国中医杂志
The American journal of Chinese medicine
作者 Kang  Bo Kyung  Kim  Mi Kyoung  Kim  So Young  Lee  Seung Jin  Choi  Young Whan  Choi  Byung Tae  Shin  Hwa Kyoung 

文献标题 钩藤对LPS刺激的BV2小胶质细胞和局灶性脑缺血小鼠的抗炎作用。
Anti-Neuroinflammatory Effects of Uncaria sinensis in LPS-Stimulated BV2 Microglia Cells and Focal Cerebral Ischemic Mice.
Anti-Neuroinflammatory Effects of Uncaria sinensis in LPS-Stimulated BV2 Microglia Cells and Focal Cerebral Ischemic Mice.

文献摘要

钩藤(Uncaria sinensis,US)长期以来被用作韩国传统药物,用于治疗心血管和中枢神经系统疾病,包括高血压和脑缺血。近年来的研究表明,超声对缺血性脑损伤具有神经保护和脑血管保护作用;然而,人们对我们的抗炎作用知之甚少。因此,本研究旨在验证US的抗炎作用。在脂多糖(LPS)刺激的小鼠BV2小胶质细胞和光血栓性皮质缺血诱导的脑损伤中,研究了US对促炎介质的抗炎作用。US正己烷提取物(HEUS)能显著抑制LPS刺激的BV2小胶质细胞一氧化氮(NO)和前列腺素E2(PGE2)的生成,抑制LPS诱导的iNOS和COX-2的表达,且呈剂量依赖性,对BV2细胞无细胞毒性。此外,HEUS显著降低促炎细胞因子TNF-α、IL-1β和IL-6的产生,抑制LPS刺激的BV2细胞NF-κB的转录活性和核转位。在一项体内研究中,高浓缩铀治疗缺血性脑损伤48小时后可显著减少梗死体积,改善神经功能,可能是通过抑制促炎细胞因子的产生。HEUS可抑制LPS刺激的促炎介质的产生,预防脑缺血损伤,提示US在预防和治疗伴有小胶质细胞活化的缺血性卒中方面具有潜在的治疗作用。


Uncaria sinensis (US) has long been used as a traditional Korean medicine to treat cardiovascular and central nervous system diseases, including hypertension and cerebral ischemia. Several recent studies have indicated that US has neuroprotective and cerebrovascular protective effects in ischemic brain injury; however, little is known about the anti-inflammatory effects of US. Therefore, the present study was designed to validate the anti-inflammatory effects of US. The anti-neuroinflammatory properties of US on pro-inflammatory mediators were investigated in lipopolysaccharide (LPS)-stimulated murine BV2 microglia and injured brains induced by photothrombotic cortical ischemia. Hexane extracts of US (HEUS) significantly suppressed the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated BV2 microglia and inhibited LPS-induced expression of iNOS and COX-2 in a dose-dependent manner without causing cytotoxicity in BV2 cells. In addition, HEUS significantly reduced the generation of pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6. Moreover, HEUS treatment inhibited the transcriptional activity and nuclear translocation of NF-κB in LPS-stimulated BV2 cells. In an in vivo study, treatment of HEUS resulted in significantly reduced infarct volume and improved neurological function 48 h after ischemic brain injury, possibly through the inhibition of the production of pro-inflammatory cytokines. HEUS inhibits LPS-stimulated production of pro-inflammatory mediators and prevents cerebral ischemic damage, suggesting that US may have therapeutic potential for the prevention and treatment of ischemic stroke accompanied by microglia activation.

Uncaria sinensis (US) has long been used as a traditional Korean medicine to treat cardiovascular and central nervous system diseases, including hypertension and cerebral ischemia. Several recent studies have indicated that US has neuroprotective and cerebrovascular protective effects in ischemic brain injury; however, little is known about the anti-inflammatory effects of US. Therefore, the present study was designed to validate the anti-inflammatory effects of US. The anti-neuroinflammatory properties of US on pro-inflammatory mediators were investigated in lipopolysaccharide (LPS)-stimulated murine BV2 microglia and injured brains induced by photothrombotic cortical ischemia. Hexane extracts of US (HEUS) significantly suppressed the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated BV2 microglia and inhibited LPS-induced expression of iNOS and COX-2 in a dose-dependent manner without causing cytotoxicity in BV2 cells. In addition, HEUS significantly reduced the generation of pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6. Moreover, HEUS treatment inhibited the transcriptional activity and nuclear translocation of NF-κB in LPS-stimulated BV2 cells. In an in vivo study, treatment of HEUS resulted in significantly reduced infarct volume and improved neurological function 48 h after ischemic brain injury, possibly through the inhibition of the production of pro-inflammatory cytokines. HEUS inhibits LPS-stimulated production of pro-inflammatory mediators and prevents cerebral ischemic damage, suggesting that US may have therapeutic potential for the prevention and treatment of ischemic stroke accompanied by microglia activation.


获取全文 10.1142/S0192415X15500639