PTEN过表达对肝癌细胞凋亡的影响。

PubMed ID
发表日期 2016年May月

原始出处 遗传学与分子研究
Genetics and molecular research : GMR
作者 Li  M F  Guan  H  Zhang  D D 

文献标题 PTEN过表达对肝癌细胞凋亡的影响。
Effect of overexpression of PTEN on apoptosis of liver cancer cells.

文献摘要

肝癌是一种常见的恶性肿瘤,生存期短,死亡率高,在我国的患病率尤其高。本研究旨在探讨过度表达10号染色体上缺失的磷酸酶和张力蛋白同源物(PTEN)基因对肝癌细胞凋亡的影响,为该病的治疗提供新的思路。实验设计包括四个治疗组,分别为转染PTEN重组质粒(HHCC+PTEN,H22+PTEN)的HHCC和H22细胞,以及转染对照质粒(HHCC+NC,H22+NC)的HHCC和H22细胞。定量pcr检测ptenmrna表达,westernblot检测蛋白水平。流式细胞术和末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法检测细胞凋亡。转染pcDNA3.1-PTEN的细胞ptenmrna表达较对照组显著增加(P<0.05)。此外,westernblotting显示,与对照组相比,治疗组PTEN蛋白表达显著升高(P<0.05)。流式细胞术显示HHCC+PTEN(约21.9%)和H22+PTEN(约41.0%)细胞凋亡率均显著高于对照组(P<0.05)。实验组与对照组细胞凋亡率差异有显著性(P<0.05)。本研究成功地将pcDNA3.1-PTEN在体外转染HHCC和H22细胞。我们认为PTEN的过度表达可以有效地抑制这些细胞的增殖,促进其凋亡。


Liver cancer is a common malignant tumor associated with a short-survival period and high-mortality rate, and its prevalence in China is particularly high. This study aimed to investigate the effect of overexpressing the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) gene on liver cancer cell apoptosis and provide new insight into the treatment of this disease. The experimental design included four treatment groups, consisting of HHCC and H22 cells transfected with PTEN recombinant plasmids (HHCC+PTEN, H22+PTEN), and those transfected with control plasmids (HHCC+NC, H22+NC). The expression of PTEN mRNA was determined by quantitative polymerase chain reaction, and protein levels were examined by western blot. Cell apoptosis was measured using flow cytometry and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling. PTEN mRNA expression in cells transfected with pcDNA3.1-PTEN was significantly increased compared to the control groups (P < 0.05). In addition, western blotting revealed PTEN protein expression in the treatment groups to be significantly elevated in comparison to control cells (P < 0.05). Flow cytometry showed that apoptosis rates of both HHCC+PTEN (approximately 21.9%) and H22+PTEN (approximately 41.0%) cells were significantly higher than those of the control groups (P < 0.05). Moreover, the difference in apoptosis rate between experimental and control groups was significant (P < 0.05). In this study, HHCC and H22 cells were successfully transfected with pcDNA3.1-PTEN in vitro. We conclude that overexpression of PTEN can effectively inhibit proliferation of these cells and promote their apoptosis.


获取全文 10.4238/gmr.15028120