钩藤乙醇提取物的扩血管作用机制。

PubMed ID
G H
发表日期 2017年Sep月

原始出处 药用食品杂志
Journal of medicinal food
作者 Loh  Yean Chun  Ch'ng  Yung Sing  Tan  Chu Shan  Ahmad  Mariam  Asmawi  Mohd Zaini  Yam  Mun Fei 

文献标题 钩藤乙醇提取物的扩血管作用机制。
Mechanisms of Action of Uncaria rhynchophylla Ethanolic Extract for Its Vasodilatory Effects.
Mechanisms of Action of Uncaria rhynchophylla Ethanolic Extract for Its Vasodilatory Effects.

文献摘要

钩藤是治疗高血压的中药方剂中的主要成分之一。以前的研究表明,美国。钩藤可能含有血管舒张介导的活性成分,特别是吲哚生物碱。因此,本研究旨在确定U。不同溶剂提取钩藤。对其信号转导机制进行了研究。美国的真实性。采用现代化的三步傅里叶变换红外光谱(FTIR)技术,包括常规的1D-FTIR、二阶导数扫描和2D相关红外光谱,对钩藤进行了鉴定。结果表明,该指纹图谱具有较好的稳定性。所用钩藤属与地图集相同。从雄性Sprague-Dawley大鼠分离的主动脉环用苯肾上腺素(PE)预限制,然后累积添加U。钩藤提取物。通过与不同受体拮抗剂的孵育,研究了PE预牵拉前的信号转导途径。总之,95%乙醇U。钩藤提取物(GT100)的EC50值为0.028 ± 0.002 mg/mL,Rmax值为101.30% ± 2.82%. 发挥其血管舒张作用的信号途径包括一氧化氮/可溶性鸟苷酰化酶/环磷酸鸟苷(NO/sGC/cGMP)和PGI2(内皮源性舒张因子)、G蛋白偶联的M3-和β2受体、通过电压控制的钙通道调节膜电位,肌醇三磷酸受体(IP3R)和除Kca通道外的所有钾通道释放胞内Ca2+。


Uncaria rhynchophylla is one of the major components included in Traditional Chinese Medicine prescriptions for hypertensive treatment. Previous studies have suggested that U. rhynchophylla might contain vasodilation-mediating active compounds, especially indole alkaloids. Hence, this study was carried out to determine the vasodilatory effects of U. rhynchophylla, which was extracted by different solvents. The most effective extract was then further studied for its signaling mechanism pathways. The authenticity of U. rhynchophylla was assured by using modernized tri-step Fourier transform infrared (FTIR), including conventional 1D FTIR, second derivative scanning combined with 2D-correlated IR spectroscopy. Results obtained proved that the fingerprint of U. rhynchophylla used was identical to the atlas. Isolated aortic rings from male Sprague-Dawley rats were preconstricted with phenylephrine (PE) followed by cumulative addition of U. rhynchophylla extracts. The signaling mechanism pathways were studied by incubation with different receptor antagonists before the PE precontraction. In conclusion, the 95% ethanolic U. rhynchophylla extract (GT100) was found to be most effective with an EC50 value of 0.028 ± 0.002 mg/mL and an Rmax value of 101.30% ± 2.82%. The signaling mechanism pathways employed for exerting its vasodilatory effects included nitric oxide/soluble guanylyl cylcase/cyclic guanosine monophosphate (NO/sGC/cGMP) and PGI2 (endothelium-derived relaxing factors), G protein-coupled M3- and β2 receptors, regulation of membrane potential through voltage-operated calcium channel, intracellular Ca2+ released from inositol triphosphate receptor (IP3R), and all potassium channels except the Kca channel.

Uncaria rhynchophylla is one of the major components included in Traditional Chinese Medicine prescriptions for hypertensive treatment. Previous studies have suggested that U. rhynchophylla might contain vasodilation-mediating active compounds, especially indole alkaloids. Hence, this study was carried out to determine the vasodilatory effects of U. rhynchophylla, which was extracted by different solvents. The most effective extract was then further studied for its signaling mechanism pathways. The authenticity of U. rhynchophylla was assured by using modernized tri-step Fourier transform infrared (FTIR), including conventional 1D FTIR, second derivative scanning combined with 2D-correlated IR spectroscopy. Results obtained proved that the fingerprint of U. rhynchophylla used was identical to the atlas. Isolated aortic rings from male Sprague-Dawley rats were preconstricted with phenylephrine (PE) followed by cumulative addition of U. rhynchophylla extracts. The signaling mechanism pathways were studied by incubation with different receptor antagonists before the PE precontraction. In conclusion, the 95% ethanolic U. rhynchophylla extract (GT100) was found to be most effective with an EC50 value of 0.028 ± 0.002 mg/mL and an Rmax value of 101.30% ± 2.82%. The signaling mechanism pathways employed for exerting its vasodilatory effects included nitric oxide/soluble guanylyl cylcase/cyclic guanosine monophosphate (NO/sGC/cGMP) and PGI2 (endothelium-derived relaxing factors), G protein-coupled M3- and β2 receptors, regulation of membrane potential through voltage-operated calcium channel, intracellular Ca2+ released from inositol triphosphate receptor (IP3R), and all potassium channels except the Kca channel.


获取全文 10.1089/jmf.2016.3804