杜仲通过抑制糖尿病小鼠肾脏晚期糖基化终产物改善糖毒性。

PubMed ID
G H
发表日期 2018年Feb月

原始出处 营养物
Nutrients
作者 Do  Moon Ho  Hur  Jinyoung  Choi  Jiwon  Kim  Mina  Kim  Min Jung  Kim  Yoonsook  Ha  Sang Keun 

文献标题 杜仲通过抑制糖尿病小鼠肾脏晚期糖基化终产物改善糖毒性。
Eucommia ulmoides Ameliorates Glucotoxicity by Suppressing Advanced Glycation End-Products in Diabetic Mice Kidney.
Eucommia ulmoides Ameliorates Glucotoxicity by Suppressing Advanced Glycation End-Products in Diabetic Mice Kidney.

文献摘要

杜仲。(EU)又名杜仲,是亚洲常用的一种治疗高血压和糖尿病的草药。尽管有证据表明EU对糖尿病有保护作用,但其对晚期糖基化终产物(AGEs)的确切作用和作用机制尚不清楚。在这项研究中,我们评估了EU对AGEs诱导的肾脏疾病的影响,并探讨了链脲佐菌素(STZ)诱导的糖尿病小鼠的潜在机制。STZ诱导的糖尿病小鼠口服EU提取物(200mg/kg)6周。EU治疗并没有改变糖尿病小鼠的血糖和糖化血红蛋白(HbA1c)水平。然而,EU治疗组的蛋白表达和乙醛酶1(Glo1)活性显著增加,Glo1可解毒AGE前体甲基乙二醛(MGO)。EU显著上调核因子红细胞2相关因子2(Nrf2)的表达,但下调AGE受体(RAGE)的表达。此外,肾脏组织的组织学和免疫组织化学分析显示,铕可减少糖尿病小鼠的高碘酸Schiff(PAS)阳性染色、AGEs和MGO积累。基于这些发现,我们得出结论,EU通过Glo1和Nrf2途径抑制AGEs的形成和RAGE的表达,减轻氧化应激,从而改善糖尿病小鼠的肾损伤。


Eucommia ulmoides Oliv. (EU), also known as Du-Zhong, is a medicinal herb commonly used in Asia to treat hypertension and diabetes. Despite evidence of the protective effects of EU against diabetes, its precise effects and mechanisms of action against advanced glycation end-products (AGEs) are unclear. In this study, we evaluated the effects of EU on AGEs-induced renal disease and explored the possible underlying mechanisms using streptozotocin (STZ)-induced diabetic mice. STZ-induced diabetic mice received EU extract (200 mg/kg) orally for 6 weeks. EU treatment did not change blood glucose and glycated hemoglobin (HbA1c) levels in diabetic mice. However, the EU-treated group showed a significant increase in the protein expression and activity of glyoxalase 1 (Glo1), which detoxifies the AGE precursor, methylglyoxal (MGO). EU significantly upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) expression but downregulated that of receptor for AGE (RAGE). Furthermore, histological and immunohistochemical analyses of kidney tissue showed that EU reduced periodic acid-Schiff (PAS)-positive staining, AGEs, and MGO accumulation in diabetic mice. Based on these findings, we concluded that EU ameliorated the renal damage in diabetic mice by inhibiting AGEs formation and RAGE expression and reducing oxidative stress, through the Glo1 and Nrf2 pathways.

Eucommia ulmoides Oliv. (EU), also known as Du-Zhong, is a medicinal herb commonly used in Asia to treat hypertension and diabetes. Despite evidence of the protective effects of EU against diabetes, its precise effects and mechanisms of action against advanced glycation end-products (AGEs) are unclear. In this study, we evaluated the effects of EU on AGEs-induced renal disease and explored the possible underlying mechanisms using streptozotocin (STZ)-induced diabetic mice. STZ-induced diabetic mice received EU extract (200 mg/kg) orally for 6 weeks. EU treatment did not change blood glucose and glycated hemoglobin (HbA1c) levels in diabetic mice. However, the EU-treated group showed a significant increase in the protein expression and activity of glyoxalase 1 (Glo1), which detoxifies the AGE precursor, methylglyoxal (MGO). EU significantly upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) expression but downregulated that of receptor for AGE (RAGE). Furthermore, histological and immunohistochemical analyses of kidney tissue showed that EU reduced periodic acid-Schiff (PAS)-positive staining, AGEs, and MGO accumulation in diabetic mice. Based on these findings, we concluded that EU ameliorated the renal damage in diabetic mice by inhibiting AGEs formation and RAGE expression and reducing oxidative stress, through the Glo1 and Nrf2 pathways.


获取全文 10.3390/nu10030265