用荧光成像法比较干性皮肤和正常皮肤角质层神经酰胺滞留的动物模型。

PubMed ID
G H
发表日期 2019年Mar月

原始出处 皮肤研究与技术:国际生物工程与皮肤学会(ISBS)和国际皮肤数字成像学会(ISDIS)的官方期刊和国际皮肤成像学会(ISSI)
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
作者 Aoki  Miku  Ogai  Kazuhiro  Kobayashi  Masato  Minematsu  Takeo  Nakatani  Toshio  Okuwa  Mayumi  Sanada  Hiromi  Sugama  Junko 

文献标题 用荧光成像法比较干性皮肤和正常皮肤角质层神经酰胺滞留的动物模型。
Comparison of ceramide retention in the stratum corneum between dry skin and normal skin using animal model with fluorescent imaging method.
Comparison of ceramide retention in the stratum corneum between dry skin and normal skin using animal model with fluorescent imaging method.

文献摘要 BACKGROUND/PURPOSE

通过保湿的皮肤护理弥补了皮肤屏障功能的不足。然而,保湿霜的使用方法并没有明确的决定。在这里,我们重点研究和审查了保留外用神经酰胺在角质层(SC)使用荧光成像方法。本研究旨在通过动物模型比较神经酰胺在正常皮肤和干性皮肤SC中的滞留情况。

METHODS

9周龄Sprague-Dawley大鼠分为正常皮肤组和干性皮肤组。干性皮肤模型组给予丙酮乙醚溶液治疗。制备荧光标记的神经酰胺溶液并应用于大鼠背部皮肤。在施用神经酰胺后0分钟和12小时采集皮肤样本。荧光标记神经酰胺在显微镜下观察。

RESULTS

正常皮肤组在用药后0分钟至12小时,神经酰胺外敷强度无明显变化。而干性皮肤模型组在用药后0分钟至12小时,神经酰胺外敷强度显著增加。

CONCLUSION

我们的研究结果显示,外用神经酰胺比正常皮肤更能穿透干燥皮肤的SC。


BACKGROUND/PURPOSE

Skin care via moisturization compensates for the lack of skin barrier function. However, moisturizer application methods are not clearly decided. Here, we focused on and examined the retention of externally applied ceramide in the stratum corneum (SC) using fluorescent imaging method. This study aimed to compare ceramide retention in the SC between normal skin and dry skin using an animal model.

METHODS

Nine-week-old Sprague-Dawley rats were divided into two groups: normal skin and dry skin model. The dry skin model group was treated with acetone-diethyl ether solution. A fluorescently labeled ceramide solution was prepared and applied to rats' back skin. Skin samples were taken at 0 minute and 12 hours after ceramide application. Fluorescently labeled ceramide was evaluated and observed under a microscope.

RESULTS

The intensity of externally applied ceramide in the normal skin group showed no significant change from 0 minute to 12 hours after application. In contrast, in the dry skin model group, the intensity of externally applied ceramide increased significantly from 0 minute to 12 hours after application.

CONCLUSION

Our findings demonstrate that the externally applied ceramide penetrated the SC of dry skin more than that of normal skin.

BACKGROUND/PURPOSE

Skin care via moisturization compensates for the lack of skin barrier function. However, moisturizer application methods are not clearly decided. Here, we focused on and examined the retention of externally applied ceramide in the stratum corneum (SC) using fluorescent imaging method. This study aimed to compare ceramide retention in the SC between normal skin and dry skin using an animal model.

METHODS

Nine-week-old Sprague-Dawley rats were divided into two groups: normal skin and dry skin model. The dry skin model group was treated with acetone-diethyl ether solution. A fluorescently labeled ceramide solution was prepared and applied to rats' back skin. Skin samples were taken at 0 minute and 12 hours after ceramide application. Fluorescently labeled ceramide was evaluated and observed under a microscope.

RESULTS

The intensity of externally applied ceramide in the normal skin group showed no significant change from 0 minute to 12 hours after application. In contrast, in the dry skin model group, the intensity of externally applied ceramide increased significantly from 0 minute to 12 hours after application.

CONCLUSION

Our findings demonstrate that the externally applied ceramide penetrated the SC of dry skin more than that of normal skin.


获取全文 10.1111/srt.12625