钩藤生物碱提取物对脂多糖致妊娠大鼠子痫前期症状及炎症的影响。

PubMed ID
G H
发表日期 2019年月

原始出处 巴西医学和生物学研究杂志
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
作者 Wu  Liang-Zhi  Xiao  Xiao-Min 

文献标题 钩藤生物碱提取物对脂多糖致妊娠大鼠子痫前期症状及炎症的影响。
Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model.
Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model.

文献摘要

过度的促炎细胞因子导致不良妊娠结局,包括先兆子痫样表型和胎儿生长受限。抗炎可能是一种有效的治疗方法。研究钩藤生物碱提取物(URE)对脂多糖(LPS)诱导的子痫前期大鼠模型的抗炎作用及改善子痫前期临床特征的作用。妊娠14~19天,每日给予LPS(1μg/kg体重/天)建立大鼠模型。妊娠14~19天,给予不同剂量的URE(35、70、140mg/kg体重/天),观察URE对蛋白尿、母性高血压、妊娠结局的影响,同时测定血清和胎盘中促炎细胞因子水平。高剂量URE(HURE)治疗可降低LPS诱导的24h平均蛋白尿和收缩压,增加胎儿重量、胎盘重量和活仔数(P<0.05)。LPS治疗组血清和胎盘IL-6、IL-1β、tnf-α、ifn-γ水平升高,HURE治疗后明显抑制(P<0.01)。在LPS诱导的子痫前期大鼠模型中,URE改善了子痫前期症状,减轻了炎症反应,提示URE的抗炎作用可能是子痫前期的一种替代疗法。


Excessive pro-inflammatory cytokines result in adverse pregnancy outcomes, including preeclampsia-like phenotypes, and fetal growth restriction. Anti-inflammation might be an effective therapy. The aim of this research was to investigate whether Uncaria rhynchophylla alkaloid extract (URE), a highly safe anti-inflammation constituent of the herb, can inhibit inflammation and improve clinical characteristics of preeclampsia in a lipopolysaccharide (LPS)-induced preeclampsia rat model. The rat model was established by daily administration of LPS (1 μg/kg body weight per day) from gestational day (GD) 14 to 19. Different doses of URE (35, 70, and 140 mg/kg body weight per day) were administered from GD 14 to GD 19. The effects of URE on proteinuria, maternal hypertension, pregnancy outcomes, as well as pro-inflammatory cytokines levels in serum and placenta were measured. High-dose URE (HURE) treatment decreased LPS-induced mean 24-h proteinuria and systolic blood pressure, and increased fetal weight, placental weight, and the number of live pups (P<0.05). Moreover, increased serum and placental levels of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and interferon-γ in the LPS-treated group were obviously inhibited after HURE administration (P<0.01). URE improved preeclampsia symptoms and mitigated inflammatory responses in the LPS-induced preeclampsia rat model, which suggests that the anti-inflammation effect of URE might be an alternative therapy for preeclampsia.

Excessive pro-inflammatory cytokines result in adverse pregnancy outcomes, including preeclampsia-like phenotypes, and fetal growth restriction. Anti-inflammation might be an effective therapy. The aim of this research was to investigate whether Uncaria rhynchophylla alkaloid extract (URE), a highly safe anti-inflammation constituent of the herb, can inhibit inflammation and improve clinical characteristics of preeclampsia in a lipopolysaccharide (LPS)-induced preeclampsia rat model. The rat model was established by daily administration of LPS (1 μg/kg body weight per day) from gestational day (GD) 14 to 19. Different doses of URE (35, 70, and 140 mg/kg body weight per day) were administered from GD 14 to GD 19. The effects of URE on proteinuria, maternal hypertension, pregnancy outcomes, as well as pro-inflammatory cytokines levels in serum and placenta were measured. High-dose URE (HURE) treatment decreased LPS-induced mean 24-h proteinuria and systolic blood pressure, and increased fetal weight, placental weight, and the number of live pups (P <0.05). Moreover, increased serum and placental levels of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and interferon-γ in the LPS-treated group were obviously inhibited after HURE administration (P<0.01). URE improved preeclampsia symptoms and mitigated inflammatory responses in the LPS-induced preeclampsia rat model, which suggests that the anti-inflammation effect of URE might be an alternative therapy for preeclampsia.


获取全文 10.1590/1414-431X20198273