环糊精修饰胶束电动毛细管色谱法同时分离和测定多毛碱和多毛碱。

PubMed ID
G H
发表日期 2020年Jan月

原始出处 植物化学分析
Phytochemical analysis : PCA
作者 Wang  Mengli  Guo  Jing  Wang  Zhiying  Zhang  Guangbin  Yu  Haixia  Chang  Ruimiao  Chen  Anjia 

文献标题 环糊精修饰胶束电动毛细管色谱法同时分离和测定多毛碱和多毛碱。
Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin-modified micellar electrokinetic capillary chromatography.
Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin-modified micellar electrokinetic capillary chromatography.

文献摘要 INTRODUCTION

粗毛碱和粗毛碱是钩藤的主要药理活性成分,在治疗阿尔茨海默病、高血压、帕金森病、潜在抗癌活性等精神和心血管疾病方面发挥着重要作用。

OBJECTIVE

建立环糊精修饰的胶束电动毛细管色谱法(CD-MEKC),用于同时分离和测定UR及其制剂中的多毛碱和多毛碱。

METHODOLOGY

通过考察影响分离的重要因素,确定了最佳方法,并将该方法成功地应用于UR及其制剂中多毛碱和多毛碱的测定。

RESULTS

最佳背景电解质(BGE)由40mM磷酸二氢钠(pH 7.0)、150mm 2,6-二甲基-β-环糊精(DM-β-CD)、3mm单-(6-乙二胺-6-脱氧)-β-环糊精(ED-β-CD)和30mM胆酸钠(SC)组成。在此条件下,在分离电压为15 kV、温度为25°C、检测波长为224 nm的条件下,在13 min内成功地分离了多毛碱和多毛碱。对于分析物,线性校准曲线在5.0-160.0μg/mL的范围内进行。检测限(LOD,S/N=3)和定量限(LOQ,S/N=10)分别为0.41,1.42μg/mL(毛苏氨酸)和0.60,2.17μg/mL(毛苏氨酸)。三种样品的回收率为97.9%~102.3%。

CONCLUSION

该方法已成功应用于乌尔及其制剂中多毛碱和多毛碱的测定。同时,为乌拉尔及其制剂的质量控制提供了有效的参考。


INTRODUCTION

Hirsutine and hirsuteine are the main pharmacological activity ingredients of Uncaria rhynchophylla (UR), playing an important role in treating mental and cardiovascular diseases, such as Alzheimer's disease, hypertension, Parkinson's disease, potential anti-cancer activities and so on.

OBJECTIVE

To develop a cyclodextrin-modified micellar electrokinetic capillary chromatography (CD-MEKC) method for the simultaneous separation and determination of hirsutine and hirsuteine from UR and its formulations.

METHODOLOGY

The optimal method was developed by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of hirsutine and hirsuteine in UR and its formulations.

RESULTS

The optimal background electrolyte (BGE) consisted of 40 mM sodium dihydrogen phosphate (pH 7.0), 150 mM 2,6-dimethyl-β-cyclodextrin (DM-β-CD), 3 mM mono-(6-ethylenediamine-6-deoxy)-β-cyclodextrin (ED-β-CD), and 30 mM sodium cholate (SC). Under these conditions, hirsutine and hirsuteine were successfully separated within 13 min at the separation voltage of 15 kV, temperature of 25°C and the detection wavelength of 224 nm. For the analytes, linear calibration curves were performed within the range 5.0-160.0 μg/mL. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were 0.41, 1.42 μg/mL for hirsutine and 0.60, 2.17 μg/mL for hirsuteine, respectively. The recoveries of three samples were from 97.9% to 102.3%.

CONCLUSION

The method was successfully applied to the determination of hirsutine and hirsuteine in UR and its formulations. Meanwhile, it provides an effective reference of the quality control of UR and its formulations.

INTRODUCTION

Hirsutine and hirsuteine are the main pharmacological activity ingredients of Uncaria rhynchophylla (UR), playing an important role in treating mental and cardiovascular diseases, such as Alzheimer's disease, hypertension, Parkinson's disease, potential anti-cancer activities and so on.

OBJECTIVE

To develop a cyclodextrin-modified micellar electrokinetic capillary chromatography (CD-MEKC) method for the simultaneous separation and determination of hirsutine and hirsuteine from UR and its formulations.

METHODOLOGY

The optimal method was developed by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of hirsutine and hirsuteine in UR and its formulations.

RESULTS

The optimal background electrolyte (BGE) consisted of 40 mM sodium dihydrogen phosphate (pH 7.0), 150 mM 2,6-dimethyl-β-cyclodextrin (DM-β-CD), 3 mM mono-(6-ethylenediamine-6-deoxy)-β-cyclodextrin (ED-β-CD), and 30 mM sodium cholate (SC). Under these conditions, hirsutine and hirsuteine were successfully separated within 13 min at the separation voltage of 15 kV, temperature of 25°C and the detection wavelength of 224 nm. For the analytes, linear calibration curves were performed within the range 5.0-160.0 μg/mL. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were 0.41, 1.42 μg/mL for hirsutine and 0.60, 2.17 μg/mL for hirsuteine, respectively. The recoveries of three samples were from 97.9% to 102.3%.

CONCLUSION

The method was successfully applied to the determination of hirsutine and hirsuteine in UR and its formulations. Meanwhile, it provides an effective reference of the quality control of UR and its formulations.


获取全文 10.1002/pca.2871