急性扁桃体炎患者口腔菌群组成的横断面调查。

PubMed ID
发表日期 2019年12月

原始出处 BMC口腔健康
BMC oral health
作者 Yeoh  Yun Kit  Chan  Man Hin  Chen  Zigui  Lam  Eddy W H  Wong  Po Yee  Ngai  Chi Man  Chan  Paul K S  Hui  Mamie 

文献标题 急性扁桃体炎患者口腔菌群组成的横断面调查。
The human oral cavity microbiota composition during acute tonsillitis: a cross-sectional survey.

文献摘要 BACKGROUND

对发炎扁桃体组织的微生物培养研究表明,链球菌、葡萄球菌和普雷沃杆菌等微生物的富集。这些分类群也在很大程度上反映在DNA测序研究进行使用组织材料。相比之下,尽管急性扁桃体炎在人类健康中起着重要作用,但人们对整个口腔微生物群对急性扁桃体炎的反应知之甚少,有证据表明其组成与口腔癌等疾病相关。此外,受试者的特殊情况(包括处方抗生素的使用和吸烟习惯)对急性扁桃体炎微生物学的影响尚不清楚。

METHODS

我们收集了43名急性扁桃体炎住院患者和165名非疾病志愿者的口腔冲洗液样本,并用16srrna基因测序方法比较了他们的微生物群落组成。我们评估了扁桃体炎、是否给受试者开抗生素、口腔脓肿的存在和他们的吸烟习惯对社区组成的影响,并确定了与扁桃体炎和吸烟相关的特定微生物类群。

RESULTS

口腔冲洗社区组成主要与疾病状态有关(扁桃体炎与非扁桃体炎),尽管其影响很小,其次是吸烟习惯。与非扁桃体炎组相比,扁桃体炎组多个普氏菌类群丰富,而非扁桃体炎组主要与奈瑟菌序列变异相关。口腔脓肿的存在对社区组成没有显著影响。扁桃体炎队列中的一部分人被开了抗生素,但我们没有观察到与抗生素消费相关的社区组成的差异。在扁桃体炎和非扁桃体炎队列中,吸烟习惯与几种梭杆菌变异体的富集有关。

CONCLUSIONS

这些发现表明,急性扁桃体炎期间口腔微生物群落发生改变,扁桃体炎期间普雷沃菌持续富集,提高了靶向干预的可能性。它也支持吸烟、梭杆菌和口腔癌之间的可能联系。


BACKGROUND

Microbial culture-based investigations of inflamed tonsil tissues have previously indicated enrichment of several microorganisms such as Streptococcus, Staphylococcus and Prevotella. These taxa were also largely reflected in DNA sequencing studies performed using tissue material. In comparison, less is known about the response of the overall oral cavity microbiota to acute tonsillitis despite their role in human health and evidence showing that their compositions are correlated with diseases such as oral cancers. In addition, the influence of subject-specific circumstances including consumption of prescription antibiotics and smoking habits on the microbiology of acute tonsillitis is unknown.

METHODS

We collected oral rinse samples from 43 individuals admitted into hospital for acute tonsillitis and 165 non-disease volunteers recruited from the public, and compared their microbial community compositions using 16S rRNA gene sequencing. We assessed the impact of tonsillitis, whether subjects were prescribed antibiotics, the presence of oral abscesses and their smoking habits on community composition, and identified specific microbial taxa associated with tonsillitis and smoking.

RESULTS

Oral rinse community composition was primarily associated with disease state (tonsillitis vs non-tonsillitis) although its effect was subtle, followed by smoking habit. Multiple Prevotella taxa were enriched in tonsillitis subjects compared to the non-tonsillitis cohort, whereas the non-tonsillitis cohort primarily showed associations with several Neisseria sequence variants. The presence of oral abscesses did not significantly influence community composition. Antibiotics were prescribed to a subset of individuals in the tonsillitis cohort but we did not observe differences in community composition associated with antibiotics consumption. In both tonsillitis and non-tonsillitis cohorts, smoking habit was associated with enrichment of several Fusobacterium variants.

CONCLUSIONS

These findings show that the oral cavity microbial community is altered during acute tonsillitis, with a consistent enrichment of Prevotella during tonsillitis raising the possibility of targeted interventions. It also supports the possible link between smoking, Fusobacteria and oral cancers.


获取全文 10.1186/s12903-019-0956-5