综合微生物组学和代谢组学分析确定灵芝孢子油对小鼠的免疫增强作用。

PubMed ID
发表日期 2020年08月

原始出处 药理学研究
Pharmacological research
作者 Wu  Xu  Cao  Jiliang  Li  Mingxing  Yao  Peifen  Li  Hongyi  Xu  Wendong  Yuan  Cheng  Liu  Juyan  Wang  Shengpeng  Li  Peng  Wang  Yitao 

文献标题 综合微生物组学和代谢组学分析确定灵芝孢子油对小鼠的免疫增强作用。
An integrated microbiome and metabolomic analysis identifies immunoenhancing features of Ganoderma lucidum spores oil in mice.

文献摘要

灵芝。例如)喀斯特。是世界范围内用于促进健康的有价值的膳食补充剂,也是治疗疲劳、免疫紊乱和癌症的药用真菌。先前的研究已经揭示了灵芝和多糖提取物的免疫增强作用,可能与肠道微生物群有关。灵芝孢子油是著名的灵芝相关产品之一。然而,很少有证据支持免疫促进活动和潜在的机制。本研究旨在探讨GLSO对小鼠的免疫增强作用。GLSO增强小鼠巨噬细胞吞噬功能和NK细胞毒性。进一步的微生物组学和代谢组学研究表明,GLSO诱导肠道微生物组的结构重排,介导多种代谢物的改变。通过聚类分析、多变量分析和相关分析发现,GLSO的免疫增强作用与几种细菌属(乳酸杆菌、土杆菌和罗姆布茨菌)和种(肠乳杆菌和再流乳杆菌)的丰度升高高度相关,葡萄球菌和幽门螺杆菌的水平降低这些结果为理解GLSO对免疫系统的调节作用提供了新的见解。


Ganoderma lucidum (Leyss. ex Fr.) Karst. is a valuable dietary supplement used worldwide for promoting health as well as a medicinal fungus for handling fatigue, immunological disorders, and cancer. Previous studies have revealed the immunoenhancing effect of G. lucidum and the polysaccharide extract, with potential involvement of gut microbiome. The oil of G. lucidum spores (GLSO)is one of the well-known G. lucidum-related products. However, there is little evidence supporting the immune promotion activity and the underlying mechanisms. The present study aims to investigate the immunoenhancing effect of GLSO in mice. GLSO enhanced macrophage phagocytosis and NK cell cytotoxicity of mice. Further microbiome and metabolomics studies showed that GLSO induced structural rearrangement of gut microbiota, mediating alterations in a wide range of metabolites. By clustering, multivariate and correlation analysis, the immunoenhancing effect of GLSO was found to be highly correlated with elevated abundance of several bacterial genera (Lactobacillus, Turicibacter and Romboutsia) and species (Lactobacillus_intestinalis and Lactobacillus_reuteri), and decreased level of Staphylococcus and Helicobacter, which resulted in the regulation of a range of key metabolites such as dopamine, prolyl-glutamine, pentahomomethionine, leucyl-glutamine, l-threonine, stearoylcarnitine, dolichyl β-d-glucosyl phosphate, etc. These results provide new insights into the understanding of the modulatory effect of GLSO on immune system.


获取全文 10.1016/j.phrs.2020.104937