钩藤及其组分对自发性高血压大鼠肝脏脂质代谢的干预作用。

PubMed ID
G H
发表日期 2020年月

原始出处 药理学前沿
Frontiers in pharmacology
作者 Tian  Zhenhua  Zhang  Shiming  Wang  Huanjuan  Chen  Zhenshan  Sun  Mengjia  Sun  Linlin  Gong  Lili  Li  Yunlun  Jiang  Haiqiang 

文献标题 钩藤及其组分对自发性高血压大鼠肝脏脂质代谢的干预作用。
Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats.
Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats.

文献摘要

钩藤。甲肝灭活素广泛用于治疗高血压。钩藤提取物及其生物活性钩藤碱和异钩藤碱可降低血压和肝细胞脂肪含量。采用UPLC-Q-Orbitrap/MS脂质组学方法研究钩藤乙醇提取物(UET)、钩藤碱(RT)和异钩藤碱(IT)对自发性高血压大鼠(SHR)的降压作用。评估肝脏的组织学变化。SHR组肝组织细胞溶解、脂肪变性。用UET、RT处理WKY、SHR大鼠,绘制脂质组分的正交投影潜伏期结构判别分析(OPLS-DA)评分图。肝脏中56种内源性代谢产物如甘油酯、甘油磷脂、不饱和脂肪酸和鞘磷脂被选为潜在的高血压相关生物标志物。为进一步探索UET降压作用的代谢靶点,采用student's t检验和相关分析方法,识别模式识别并筛选出有意义的代谢产物。在所有接受UET、RT和IT治疗的SHR组中都观察到了相似且持续的血压降低,而与未经治疗的SHR相比,代谢产物谱受到轻微干扰。进一步分析表明,RT和IT中只有少数共同成分,尽管代谢途径不同,但其降压作用相似。这些结果有助于理解异构体成分在不同靶点下显示降压作用的机制。


Uncaria rhynchophylla (Miq.) Miq. ex Havil is widely used in the treatment of hypertension. The Uncaria extract and its bioactives, rhynchophylline and isorhynchophylline, reduced the blood pressure and fatty content in liver cells. In the present study, the antihypertensive effects of Uncaria ethanol extract (UET), rhynchophylline (RT) and isorhynchophylline (IT) were investigated in spontaneously hypertensive rats (SHR) using UPLC-Q-Orbitrap/MS based lipidomics approach. Histological changes in the liver were evaluated. Cytolysis and fatty degeneration in the liver tissues were observed in the SHR group. Lipid species in WKY, SHR treated with UET, RT, and IT were plotted to obtain the Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) score plots. Fifty-six endogenous metabolites in the liver such as glycerides, glycerophospholipids, unsaturated fatty acids, and sphingomyelins were selected as potential hypertension associated biomarkers. In order to further explore the metabolite targets of UET for antihypertensive, student's t test and correlation analysis were performed to recognize the pattern recognition and to select the significant metabolites. Similar and prolonged reduction in blood pressure was observed in all SHR groups treated with UET, RT, and IT, while the metabolite profiles were perturbed slightly compared to that of the untreated SHR. Further analysis showed that only a few common components were observed in both RT and IT, which showed similar antihypertensive effect in spite of the distinct metabolic pathways. These results help in understanding the mechanisms of isomeric ingredients in exhibiting the antihypertensive effect but with different targets.

Uncaria rhynchophylla (Miq.) Miq. ex Havil is widely used in the treatment of hypertension. The Uncaria extract and its bioactives, rhynchophylline and isorhynchophylline, reduced the blood pressure and fatty content in liver cells. In the present study, the antihypertensive effects of Uncaria ethanol extract (UET), rhynchophylline (RT) and isorhynchophylline (IT) were investigated in spontaneously hypertensive rats (SHR) using UPLC-Q-Orbitrap/MS based lipidomics approach. Histological changes in the liver were evaluated. Cytolysis and fatty degeneration in the liver tissues were observed in the SHR group. Lipid species in WKY, SHR treated with UET, RT, and IT were plotted to obtain the Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) score plots. Fifty-six endogenous metabolites in the liver such as glycerides, glycerophospholipids, unsaturated fatty acids, and sphingomyelins were selected as potential hypertension associated biomarkers. In order to further explore the metabolite targets of UET for antihypertensive, student's t test and correlation analysis were performed to recognize the pattern recognition and to select the significant metabolites. Similar and prolonged reduction in blood pressure was observed in all SHR groups treated with UET, RT, and IT, while the metabolite profiles were perturbed slightly compared to that of the untreated SHR. Further analysis showed that only a few common components were observed in both RT and IT, which showed similar antihypertensive effect in spite of the distinct metabolic pathways. These results help in understanding the mechanisms of isomeric ingredients in exhibiting the antihypertensive effect but with different targets.


获取全文 10.3389/fphar.2020.00910