人类免疫缺陷病毒体外感染和灭活自然杀伤细胞。

PubMed ID
发表日期 1988年May月

原始出处 人体病理学
Human pathology
作者 Robinson  W E  Mitchell  W M  Chambers  W H  Schuffman  S S  Montefiori  D C  Oeltmann  T N 

文献标题 人类免疫缺陷病毒体外感染和灭活自然杀伤细胞。
Natural killer cell infection and inactivation in vitro by the human immunodeficiency virus.

文献摘要

用人免疫缺陷病毒(HIV)的淋巴结病相关病毒(LAV)体外感染,消除了健康献血者外周血白细胞在白细胞介素-2条件培养基中的细胞溶解活性。虽然病毒抗原在感染后14天才在培养细胞中表达,但细胞溶解活性在感染后3天就丧失了。细胞溶解功能的丧失不是受感染细胞或未受感染细胞释放抑制因子的结果,因为无论是受感染细胞还是CEM细胞培养物的上清液对未感染细胞的功能都没有任何抑制作用。培养14天后,表达leu11b的淋巴细胞也能通过免疫荧光法表达HIV抗原。这些结果表明,自然杀伤(NK)细胞,如leu11b的表达所定义的,在体外被HIV感染,而溶血功能的丧失很可能是这种感染的直接后果。


Cytolytic activity of human mononuclear peripheral blood leukocytes from healthy donors, cultured in interleukin-2 conditioned medium, was abrogated by in vitro infection with the lymphadenopathy associated virus (LAV) isolate of the human immunodeficiency virus (HIV). Although viral antigens are not expressed in cultured cells until 14 days postinfection, cytolytic activity was lost as early as 3 days after infection. Loss of cytolytic function was not a result of the release of suppressive factors from either infected cells or uninfected CEM cells since supernatants from neither infected cultures nor CEM cell cultures had any inhibitory effects on the function of uninfected cells. Cultured lymphocytes expressing Leu 11b were also shown to express HIV antigens via immunofluorescence after 14 days in culture. These results suggest that natural killer (NK) cells, as defined by expression of Leu 11b, were infected by HIV in vitro and the loss of lytic function was likely a direct consequence of that infection.


获取全文 10.1016/s0046-8177(88)80200-4