用免疫学方法测定人脑膜瘤细胞溶质中的雌激素和孕激素受体。

PubMed ID
发表日期 1987年Jun月

原始出处 国际临床化学杂志
Clinica chimica acta; international journal of clinical chemistry
作者 Blankenstein  M A  van der Meulen-Dijk  C  Thijssen  J H 

文献标题 用免疫学方法测定人脑膜瘤细胞溶质中的雌激素和孕激素受体。
Assay of oestrogen and progestin receptors in human meningioma cytosols using immunological methods.

文献摘要

采用Scatchard图放射配基结合法和单克隆抗体酶免疫法测定脑膜瘤细胞液中雌激素(ER)和孕激素受体(PR)。为了进行比较,我们使用了人类乳腺癌组织。两种分析的结果都非常一致。对于人类乳腺癌,两种方法测定的受体水平显示出非常显著的差异(ER:r=0.96;n=74和PR:r=0.95;n=19)。此外,对于脑膜瘤细胞溶胶,两种检测结果之间也有很好的一致性。因此,大多数脑膜瘤缺乏雌激素受体,但含有显著的孕激素受体浓度。酶免疫分析法测定的脑膜瘤孕激素受体水平与Scatchard图分析结果有良好的相关性。对数据进行对数变换后,回归线为:PR(EIA)=0.83 X PR(Scatchard)+0.36(r=0.917;n=24)。抗孕激素受体的单克隆抗体识别脑膜瘤中的孕激素结合物,进一步表明脑膜瘤中的孕激素结合发生在真正的受体上。


Oestrogen (ER) and progestin receptors (PR) were assayed in human meningioma cytosol by radioligand binding assay with Scatchard plot analysis and by monoclonal antibody based enzyme immunoassays. For comparison, human breast cancer tissues were used. Results of both assays agreed very well. For human breast cancer, receptor levels assayed with both methods showed a highly significant (ER: r = 0.96; n = 74 and PR: r = 0.95; n = 19). Also for meningioma cytosols a good agreement was observed between the result of both assays. Thus, most meningiomas were devoid of ER but contained significant concentrations of PR. PR levels in meningioma determined with the enzyme immunoassay correlated well with those found by Scatchard plot analysis. After logarithmic transformation of the data the regression line was; PR(EIA) = 0.83 X PR(Scatchard) + 0.36 (r = 0.917; n = 24). The recognition of the progestin binder in meningioma by a monoclonal antibody against the progestin receptor is a further indication that progestin binding in meningioma occurs to a true receptor.


获取全文 10.1016/0009-8981(87)90162-8