表面工程立方体作为一种新型疫苗佐剂,在体内调节天然免疫和提高适应性免疫。

PubMed ID
发表日期 2020年月

原始出处 国际纳米医学杂志
International journal of nanomedicine
作者 Liu  Zhenguang  Yu  Lin  Gu  Pengfei  Bo  Ruonan  Xu  Shuwen  Wusiman  Adelijiang  Liu  Jiaguo  Hu  Yuanliang  Wang  Deyun 

文献标题 表面工程立方体作为一种新型疫苗佐剂,在体内调节天然免疫和提高适应性免疫。
Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo.

文献摘要 Objective

最近的研究揭示了立方体的佐剂活性及其作为抗原传递系统的潜在用途。为了进一步提高立方体的佐剂活性,本研究在立方体表面修饰了两种阳离子聚合物。

Methods

本文通过在灵芝多糖立方体(GLPC)表面放置十六烷基三甲基溴化铵(CTAB)和聚二烯丙基二甲基氯化铵(PDDAC)两种分子,研究了表面化学对其佐剂活性的影响。

Results

CTAB-或PDDAC修饰的GLPC可显著促进体液和细胞免疫应答,并通过树突状细胞(dc)的强烈激活促进CD3+CD4+或CD3+CD8+T细胞的增殖。PDDAC修饰的GLPC增强的免疫应答可能与DCs向引流淋巴结的成熟、脾脏和血清细胞因子的活化有关。

Conclusion

PDDAC修饰有利于增强体液和细胞免疫应答,提示PDDAC-GLPC-OVA有可能成为疫苗的潜在佐剂。


Objective

Recent studies have revealed the adjuvant activity of cubosomes and their potential utility as an antigen delivery system. In this study, to further enhance the adjuvant activity of cubosomes, two cationic polymers are modified on the surface of cubosomes.

Methods

Here, we exploit the effects of surface chemistry on the adjuvant activity of Ganoderma lucidum polysaccharide cubosomes (GLPC) by placing two kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB) and poly(diallydimethyl ammonium chloride) (PDDAC), on their surface.

Results

CTAB- or PDDAC-modified GLPC were found to significantly promote humoral and cellular immune responses, as well as the proliferation of CD3+ CD4+ or CD3+ CD8+T cells through the powerful activation of dendritic cells (DCs). The enhanced immune responses of PDDAC-modified GLPC might be attributed to the maturation of DCs into draining lymph nodes and the activation of spleen and cytokines in serum.

Conclusion

PDDAC modification is beneficial for enhancing humoral and cellular immune response, suggesting that PDDAC-GLPC-OVA has the ability to be a potential adjuvant for vaccine.


获取全文 10.2147/IJN.S266165