杜仲、蒺藜抗高血压作用机制的研究——基于肠道菌群和代谢组学分析。

PubMed ID
G H
发表日期 2020年月

原始出处 国际生物医学研究所
BioMed research international
作者 Qi  Ying-Zi  Yang  Xue-Song  Jiang  Yue-Hua  Shao  Lin-Lin  Jiang  Ling-Yu  Yang  Chuan-Hua 

文献标题 杜仲、蒺藜抗高血压作用机制的研究——基于肠道菌群和代谢组学分析。
Study of the Mechanism Underlying the Antihypertensive Effects of Eucommia ulmoides and Tribulus terrestris Based on an Analysis of the Intestinal Microbiota and Metabonomics.
Study of the Mechanism Underlying the Antihypertensive Effects of Eucommia ulmoides and Tribulus terrestris Based on an Analysis of the Intestinal Microbiota and Metabonomics.

文献摘要

杜仲和蒺藜的联合应用在临床上已有数千年的历史,但其作用机制尚未阐明。本研究试图探讨肠道微生物群和粪便代谢在老年自发性高血压大鼠(shr)对ET治疗高血压反应中的作用。将14只18月龄雄性自发性高血压大鼠随机分为ET组和SHR组,7只同龄Wistar-Kyoto(WKY)大鼠作为对照组。ET组灌胃给予ET 1.0g/kg/d,共42d,shr和WKY大鼠灌胃等量生理盐水。采用16srrna测序和GC-MS/MS分析肠道菌群和粪便代谢。ET治疗shr可稳定降压,改善结肠组织形态,改变不平衡菌群的结构和组成。根据Spearman相关分析,ET处理增加了真细菌的丰度,真细菌可能是ET的靶微生物之一,并且与α-生育酚、鹅去氧胆酸和脱氧胆酸的水平呈负相关。肠道微生物群的变化影响SHR的粪便代谢模式。八个潜在的生物标志物被确定主要富集在ABC转运蛋白、苯丙氨酸代谢、癌症中心碳代谢、嘌呤代谢和蛋白质消化吸收。相关分析表明,ET组真杆菌的丰度与α-生育酚、鹅去氧胆酸和脱氧胆酸水平的下降高度相关。提示ET具有良好的抗高血压作用,其机制可能与肠道菌群及其有益代谢产物有关。本研究结果有助于阐明ET的降压机制。


The combination of Eucommia ulmoides and Tribulus terrestris (ET) has been widely utilized in clinical practice for thousands of years, but the mechanism underlying its efficacy has not been elucidated to date. This study attempted to investigate the role played by the intestinal microbiota and fecal metabolism in the response of elderly spontaneous hypertensive rats (SHRs) to ET administration as a treatment for hypertension. Fourteen male spontaneously hypertensive rats (SHRs, 18 months old) were randomly divided into an ET group and an SHR group, and 7 Wistar-Kyoto (WKY) rats of the same age were employed as the control group. The ET group was intragastrically administered 1.0 g/kg/d ET for 42 days, and SHRs and WKY rats were administered an equal amount of normal saline intragastrically. The intestinal microbiota and fecal metabolism were analyzed by 16S rRNA sequencing and the GC-MS (gas chromatography-mass spectrometry)/MS assay. ET treatment decreased blood pressure steadily, improved the colonic tissue morphology, and changed the structure and composition of the imbalanced microbiota in SHRs. Specifically, ET treatment increased the abundance of Eubacterium, which might be one of the target microbes for ET, and had a negative correlation with the levels of α-tocopherol, chenodeoxycholic acid, and deoxycholic acid according to the Spearman correlation analysis. The change in the intestinal microbiota affected the fecal metabolic pattern of SHRs. Eight potential biomarkers were determined to be primarily enriched in ABC transporters, phenylalanine metabolism, central carbon metabolism in cancer, purine metabolism, and protein digestion and absorption. The correlation analysis demonstrated that the abundance of Eubacterium and the decreased levels of α-tocopherol, chenodeoxycholic acid, and deoxycholic acid in the ET group were highly correlated. Our results suggest that ET has a good antihypertensive effect, which may be driven by the intestinal microbiota and their beneficial metabolites. The results of this study may help to elucidate the antihypertensive mechanism of ET.

The combination of Eucommia ulmoides and Tribulus terrestris (ET) has been widely utilized in clinical practice for thousands of years, but the mechanism underlying its efficacy has not been elucidated to date. This study attempted to investigate the role played by the intestinal microbiota and fecal metabolism in the response of elderly spontaneous hypertensive rats (SHRs) to ET administration as a treatment for hypertension. Fourteen male spontaneously hypertensive rats (SHRs, 18 months old) were randomly divided into an ET group and an SHR group, and 7 Wistar-Kyoto (WKY) rats of the same age were employed as the control group. The ET group was intragastrically administered 1.0 g/kg/d ET for 42 days, and SHRs and WKY rats were administered an equal amount of normal saline intragastrically. The intestinal microbiota and fecal metabolism were analyzed by 16S rRNA sequencing and the GC-MS (gas chromatography-mass spectrometry)/MS assay. ET treatment decreased blood pressure steadily, improved the colonic tissue morphology, and changed the structure and composition of the imbalanced microbiota in SHRs. Specifically, ET treatment increased the abundance of Eubacterium, which might be one of the target microbes for ET, and had a negative correlation with the levels of α-tocopherol, chenodeoxycholic acid, and deoxycholic acid according to the Spearman correlation analysis. The change in the intestinal microbiota affected the fecal metabolic pattern of SHRs. Eight potential biomarkers were determined to be primarily enriched in ABC transporters, phenylalanine metabolism, central carbon metabolism in cancer, purine metabolism, and protein digestion and absorption. The correlation analysis demonstrated that the abundance of Eubacterium and the decreased levels of α-tocopherol, chenodeoxycholic acid, and deoxycholic acid in the ET group were highly correlated. Our results suggest that ET has a good antihypertensive effect, which may be driven by the intestinal microbiota and their beneficial metabolites. The results of this study may help to elucidate the antihypertensive mechanism of ET.


获取全文 10.1155/2020/4261485