倍他定灭活人体免疫缺陷病毒。

PubMed ID
发表日期 1987年Oct月

原始出处 感染控制:IC
Infection control : IC
作者 Kaplan  J C  Crawford  D C  Durno  A G  Schooley  R T 

文献标题 倍他定灭活人体免疫缺陷病毒。
Inactivation of human immunodeficiency virus by Betadine.

文献摘要

人类免疫缺陷病毒(HIV)是获得性免疫缺陷综合征(AIDS)的病因,用倍他定(聚维酮碘)溶液或倍他定手术擦剂进行治疗。HIV失活分析采用病毒逆转录酶法或观察HIV感染的H-9、T细胞培养产生的细胞病变效应。在不同时间间隔(立即旋涡至10分钟)内,完全灭活HIV的最小有效剂量为0.25%。在这些实验中使用的HIV储备的滴度(每毫升10(5)TCID50)高于临床标本中通常检测到的量。我们的研究结果为临床或实验室使用聚维酮碘作为抗HIV的局部防腐剂提供了理论依据。


Human immunodeficiency virus (HIV), the etiological agent of the acquired immunodeficiency syndrome (AIDS), was treated with either Betadine (povidone-iodine) Solution or Betadine Surgical Scrub. HIV inactivation was analyzed using the viral reverse transcriptase assay or by observing the cytopathic effect produced in HIV-infected, H-9, T-cell cultures. The minimum effective Betadine dose was 0.25% for complete inactivation of HIV that was treated for various time intervals (immediate vortex to ten minutes). The titer of HIV stocks used in these experiments (10(5) TCID50 per mL) was greater than amounts generally detected in clinical specimens. Our results provide a rationale for the use of povidone-iodine as a topical antiseptic against HIV in the clinic or laboratory.


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