用合成雌激素反应元件(ERE)带移法检测脑膜瘤中的雌激素受体样蛋白。

PubMed ID
发表日期 1993年Aug月

原始出处 英国癌症杂志
British journal of cancer
作者 Koehorst  S G  Jacobs  H M  Thijssen  J H  Blankenstein  M A 

文献标题 用合成雌激素反应元件(ERE)带移法检测脑膜瘤中的雌激素受体样蛋白。
Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).

文献摘要

当使用配体结合或酶免疫分析时,脑膜瘤富含孕激素受体(PR),而雌激素受体(ER)几乎无法检测。在脑膜瘤中检测到一种能与雌激素反应元件结合的蛋白。抗ER单克隆抗体ER-P31直接作用于ER的A/B结构域,抑制ER与ER的结合,表明结合蛋白为ER样蛋白。


When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.


获取全文 10.1038/bjc.1993.330